Expression of cytokines and regulator transcription factors of CD4+ subsets in patients with pancreatic cancer and its clinical significance" />
胰腺癌患者外周血CD4<sup>+</sup>T亚群细胞因子及转录因子的表达及其临床意义
Expression of cytokines and regulator transcription factors of CD4+ subsets in patients with pancreatic cancer and its clinical significance
LIU Yan-fang 1,2, FU Min 1, LIU Hai-bing 3, YIN Liang 4 , WANG Dong-qing 2,5
(1. Department of Central Laboratory, Affiliated People′s Hospital of Jiangsu University, Zhenjiang Jiangsu 212002; 2. School of Medicine, Jiangsu University, Zhenjiang Jiangsu 212013; 3. Department of Clinical Laboratory, Affiliated People′s Hospital of Jiangsu University, Zhenjiang Jiangsu 212002; 4. Department of General Surgery, Affiliated People′s Hospital of Jiangsu University, Zhenjiang Jiangsu 212002; 5. Department of Radiology, Affiliated Hospital of Jiangsu University, Zhenjiang Jiangsu 212001, China)
Objective: To investigate the cytokines and master regulator transcription factors of CD4+ subsets in pancreatic cancer patients. Methods: Specimens of peripheral blood were collected from 27 pancreatic cancer patients and 30 healthy controls. The serum levels of IFN-γ, IL-2, IL-4, IL-17, TGF-β and IL-10 were determined by ELISA. The mRNA expression level of T-bet, Gata-3, RORγt and Foxp3 were detected by real time-PCR. Results: Compared with healthy controls, the level of IFN-γ, IL-2 and T-bet mRNA were markedly decreased in pancreatic cancer patients(P<0.05), but the level of IL-4, IL-17, TGF-β, IL-10 and mRNA expression level of Gata-3, RORγt and Foxp3 were significantly increased in pancreatic cancer patients(all P<0.05).Conclusion: Pancreatic cancer patients showed lower levels of Th1 cytokines and master regulator transcription factors, higher levels of Th2, Th17 and Tregs cytokines and master regulator transcription factors; Th2, Th17 and Treg cells may synergistically promote the development of pancreatic cancer disease.
