(1. Department of Clinical Laboratory, Affiliated Hospital of Jiangsu University, Zhenjiang Jiangsu 212001; 2. School of Medicine, Jiangsu University, Zhenjiang Jiangsu 212013, China)
Abstract：Objective: To compare the detection efficacy of 6 universal primers in detecting 9 different types of human papillomavirus (HPV) and provide a quick and easy screening method for clinical studies. Methods: We collected 954 female cervical exfoliated cell samples, then detected and classified the HPVDNA with HPV typing kit. Then we used 6 pairs of universal primers （①GP5/GP6，②GP5+/GP6+，③CP-Ⅰ/CP-ⅡS，④CP-I/CP-ⅡG，⑤external：MY09/MY11，internal：GP5+/GP6+，⑥SPF1/GP6++）to detect HPVDNA positive samples by PCR and compared their detection effect in 9 types of HPV-DNA. Results: A total of 263 HPV-DNA positive samples were detected from 954 specimens with HPV typing kit， the HPV infection rate was 27.57%. In the HPVDNA positive samples, the positive cases detected by SPF1/GP6++ was 223, and the detection rate was 84.79%. But 207 positive cases were detected by nested PCR primers (external: MY09/MY11, internal: GP5+/GP6+), the corresponding detection rate was 78.71%. The two universal primers above were able to detect all 9 HPVDNA types. Conclusion: The universal primer SPF1/GP6++ has the best detection efficacy, but the synthesis cost is higher because of containing hypoxanthine. The nest PCR primer (external: MY09/MY11, inner:GP5+/GP6+) has the second highest detection efficacy, and the synthesis cost is lower. Therefore, we may select suitable universal primers according to the actual situation in HPV detection.
收稿日期: 2019-05-21
基金资助:
江苏省2018年度预防医学科研课题（Y2018108）; 镇江市社会发展项目（SH2017024）
作者简介: 吴瑶（1993—），女，硕士研究生
引用本文:
吴瑶1， 吴亮2， 阴晴1， 周亚玲1， 何蕾1， 戴晓玥2， 夏雯2， 许化溪2. 6对通用引物检测不同型别人乳头瘤病毒效果分析[J]. 江苏大学学报:医学版, 2019, 29(05): 405-409.
WU Yao1, WU Liang2, YIN Qing1, ZHOU Ya-ling1, HE Lei1,DAI Xiao-yue2, XIA Wen2, XU Hua-xi2. Detection efficacy of 6 universal primers in different types of papillomavirus
. Journal of Jiangsu University(Medicine Edition), 2019, 29(05): 405-409.
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2019, Vol. 29 
