Abstract：Objective: To study the effect of 17β-estradiol on the activity of Schwann cells in rats and the repair effect of 17βestradiol on transection injury of rat sciatica. Methods: Different concentrations of 17β-estradiol solution was prepared, and the proliferation of Schwann cells was detected by CCK8 kit to select the optimal concentration; the expression of estrogen receptor on Schwann cells was determined by Western blotting. Schwann cells were divided into two groups, control group（routinely cultured） and 17β-estradiol group(100 nmol/L); and the migration ability of Schwann cells was detected by Transwell test and scratch test. The mRNA expression of brain-derived neurotrophic factor(BDNF), ciliary neurotrophic factor(CNTF), glial cellderived neurotrophic factor(GDNF), nerve growth factor(NGF) and vascular endothelial growth factor(VEGF) were quantitatively detected by qRTPCR. A certain concentration of 17β-estradiol was administered by intraperitioneal injection to observe the repair of transection injury of sciatica nerve in male SD rats. Results: Compared with the control group, 17β-estradiol of 100 nmol/L increased the proliferation of Schwann cells, promoted the expression of estrogen receptors on Schwann cells, and increased the migration ability of Schwann cells, the neurotrophic factor BDNF, CNTF, GDNF and NGF mRNA levels were increased(all P<0.05). 17β-estradiol could promote the recovery of motor and sensory function after sciatic nerve injury in rats, and delay the atrophy of the gastrocnemius (all P<0.05). Conclusion: 17β-estradiol may exert a series of biological effects by binding to estrogen receptors on Schwann cells, promoting the recovery of motor and sensory functions after peripheral nerve injury.
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2019, Vol. 29 
