Abstract：Objective: To investigate the effects of morroniside on the differentiation, phenotype and function of murine bonemarrow derived dendritic cells (BMDCs) in vitro. Methods: Primary murine BMDCs were prepared and divided into four groups: normal control group, morronisidetreated group, LPStreated group and morroniside+LPStreated group. The changes of BMDCs morphology and fluorescence intensity of CFSE were confirmed respectively with light microscopy and flow cytometry (FCM) . The phenotype and functional maturation of BMDCs and activation of antigenspecific CD4+ T cell proliferation and differentiation under influence of morroniside were evaluated by FCM. ELISA was used for analyzing the protein levels of IL6/IL12 in supernatant of cultured BMDCs. Results: Compared with the normal control group, there was no significant difference in the proliferation efficiency of BMDCs induced differentiation after morroniside treatment. Compared with the LPStreated group, morroniside combined with LPS could upregulated the expression of CD80,CD86, MHCⅠ and MHCⅡ molecules and promoted the IL12 secretion on BMDCs. Furthermore, morroniside treatment promoted activation,proliferation and differentiation priming capability on OVA323-339 antigenspecific T cells of BMDCs. Conclusion: Morroniside effectively enhances the maturation of BMDCs and upregulates the antigenpresenting function of OVAspecific CD4+ T cells in vitro.
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2019, Vol. 29 
