Abstract：Objective: To investigate the effects of myeloid-related protein 14(MRP14) overexpression on the proliferation and thyroid hormones secretion of human thyroid epithelial cells. Methods: Immunohistochemical staining was used to detect the expression of MRP14 protein in thyroid tissues of patients with simple Goiter and Hashimoto′s Thyroiditis(HT); human thyroid follicular epithelial cells Nthy-ori 3-1 were transiently transfected with MRP14 gene overexpression plasmids or corresponding control to construct thyroid follicular epithelial cell lines with MRP14 overexpression. Cells and supernatant were collected after 48 h of transfection, and the transfection effect of MRP14 was detected by fluorescence quantitative realtime PCR, the cell proliferation was determined with MTT method and the concentration of supernatant free thyroid(FT4) were detected by chemiluminescence immunoassay. Results: Compared with the simple Goiter, MRP14 protein expression in Hashimoto′s Thyroiditis tissues was significantly increased. After successful transfection of MRP14 gene, the number of Nthy-ori 3-1 cells and the FT4 concentration were decreased in the MRP14 overexpression group compared with the control group(all P＜0.01). Conclusion: MRP14 overexpression in the thyroid follicular epithelial cell lines was successfully constructed. MRP14 protein may be involved in the occurrence and development of HT by inhibiting the proliferation of thyroid cells and the secretion of thyroid hormones.
［Key words］myeloid-related protein 14; proliferation; free thyroxine; Hashimoto′s Thyroiditis
2019, Vol. 29 
