Abstract：Objective: To explore the effect of ginkgol C171 on the antitumor drug resistance and apoptosis of cisplatin resistant lung adenocarcinoma (A549/DDP) cells. Methods: A549 and A549/DDP cells were cultured in vitro, and MTT assay was used to detect the drug resistance of A549/DDP cells and the optimal concentration of cisplatin. After treatment with cisplatin of the aboveoptimal concentration and various concentrations of ginkgol C171 (0,10, 20, 40 mg/L), the migration, invasion, resistancerelated proteins and apoptosis rate of A549/DDP cells were detected by Transwell assay, Western blotting and flow cytometry, respectively. Results: MTT result showed the resistance of A549/DDP cells was 5.8 times vs A549 cells, and the optimal concentration of cisplatin was 4 mg/L. Compared with the control group, Transwell assay demonstrated that the migration and invasion of A549/DDP cells were significantly inhibited in ginkgol C171 at different concentrations and cisplatin combination groups(P<0.05). Western blotting showed that the expressions of Nrf2, Mrp1 and antiapoptotic protein Bcl2 were inhibited(P<0.05), while apoptotic protein Bax and Keap1 protein were increased(P<0.01) when A549/DDP cells were cotreated with different concentrations of ginkgol C171 and cisplatin. Cytometry demonstrated that the apoptosis rates of A549/DDP cells were increased significantly by different concentrations of ginkgol C171 and cisplatin cotreatment groups(P<0.05). Conclusion: Ginkgol C171 could reverse the resistance of A549/DDP cells to cisplatin in vitro，and promote apoptosis.
收稿日期: 2017-10-16
基金资助:
国家自然科学基金资助项目(81372404)；江苏大学大学生科研立项(16A511；16A513)
通讯作者:
李月英(通讯作者)，副教授，硕士生导师，E-mail: yyli@ujs.edu.cn
作者简介: 董燕(1992—)，女，硕士研究生
引用本文:
董燕1， 李月英1， 张心驰1， 杨小明2， 厉琳杰1， 马文斌1. 银杏酚C171与顺铂联用逆转A549/DDP细胞对顺铂的耐药性[J]. 江苏大学学报:医学版, 2018, 28(02): 93-98.
DONG Yan1， LI Yue-ying1, ZHANG Xin-chi1, YANG Xiao-ming2, LI lin-jie1, MA Wen. Ginkgol C171 and cisplatin co-treatment reversed A549/DDP cells resistance to cisplatin. Journal of Jiangsu University(Medicine Edition), 2018, 28(02): 93-98.
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2018, Vol. 28 
