Intrathecal injection of cyproheptadine ameliorates bone cancer pain in mice
HANG Li-hua1, WEI Shi-you1, XU Zhen-kai1, CAI Yue-jiao1, LI Shu-na2, PENG Sheng3
（1. Department of Anesthesiology, Kunshan Hospital Affiliated to Jiangsu University, Kunshan Jiangsu 215300; 2. Department of Otorhinolaryngology, Xinhua Hospital Affiliated to Shanghai Jiaotong University School of Medicine, Shanghai 200092; 3. Department of Anesthesiology, Seventh People′s Hospital of Shanghai University of Traditional Chinese Medicine, Shanghai 200137, China )
Abstract：Objective: To investigate the effects of intrathecal injection of cyproheptadine, an inhibitor of SET domain containing lysine methyltransferase 7/9(SET7/9), on paw withdrawal mechanical threshold (PWMT) and the expression of SET7/9 in spinal dorsal horn of bone cancer pain (BCP) mice. Methods: Eightyeight male C3H/HeJ mice were selected， weighing 20-25 g. The experiments divided into two parts. Part I, mice were randomly divided into sham and BCP groups. Established a mouse model of bone cancer pain, NCTC 2472 cancer cells were inoculated into the intramedullary space of the mice left femur. The changes of PWMT were measured at l d before operation and 5, 7, 12, 15 d after operation(n=8)， and the spinal expression of SET7/9 at l d before operation and 15 d after operation (n=4) was also observed. PartⅡ, mice were randomly divided into sham, BCP, BCP+cyproheptadine 10 nmol, BCP+cyproheptadine 20 nmol, BCP+SET7/9 0.2 μg, BCP+SET7/9 0.2 μg+cyproheptadine 20 nmol, BCP+ chlorpheniramine 200 μg groups. The changes of PWMT were measured at 0 h before and 6 h after intrathecal injection(n=8) were measured in all groups, and the expression of SET7/9 in the spinal dorsal horn was measured by western blot analysis at 0 h before and 6 h after intrathecal injection in BCP and BCP+cyproheptadine 20 nmol groups(n=4). Results: The PWMT was significantly reduced 7-15 d after inoculation of cancer cells(P<0.05 or 0.01), and the spinal SET7/9 was significantly increased 15d after inoculation of cancer cells(P<0.01). Compared with BCP group, the PWMT was significantly increased 3 h after intrathecal in BCP+cyproheptadine 10 nmol group and BCP+cyproheptadine 20 nmol group. The expression of spinal SET7/9 was significantly decreased in BCP+cyproheptadine 20 nmol group. Conclusion: Intrathecal cyproheptadine could significantly increase PWMT and reduce the expression of SET7/9 in spinal dorsal injection of horn in 15 d BCP mice. Spinal SET7/9 may participate in the development of bone cancer pain in mice.
收稿日期: 2018-01-04
作者简介: 杭黎华（1974—），男，江苏丹阳人，博士，主任医师，主要从事疼痛的基础与临床研究。
引用本文:
杭黎华1， 卫世有1， 徐振锴1， 蔡玥娇1， 李姝娜2， 彭生3. 鞘内注射赛庚啶缓解小鼠骨癌痛[J]. 江苏大学学报:医学版, 2018, 28(02): 140-143,184.
HANG Li-hua1, WEI Shi-you1, XU Zhen-kai1, CAI Yue-jiao1, LI Shu-na2, PENG Sheng3. Intrathecal injection of cyproheptadine ameliorates bone cancer pain in mice
. Journal of Jiangsu University(Medicine Edition), 2018, 28(02): 140-143,184.
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2018, Vol. 28 
