Establishment of enterovirus 71-infected ICR neonatal mice model
QIN Yan-yan1, LU Jie1, LI Ji-lin1, LIU Hai-bing2, MAO Ling-xiang2, SHAO Qi-xiang1, CHEN Jian-guo2
(1. School of Medicine, Jiangsu University, Zhenjiang Jiangsu 212013; 2. Department of Clinical Laboratory, the Affiliated People′s Hospital of Jiangsu University, Zhenjiang Jiangsu 212002, China)
Abstract：Objective: To establish an EV71 infection model in ICR neonatal mice and provide a practical animal model for evaluation of EV71 vaccines and antiviral drugs. Methods: The 1day ICR mice were infected with the EV71 G03 virus strain through intracerebroventricular injection to establish animal models. After routine euthanizingly killed animals, their various organs and tissues were collected to perform RTPCR analysis of the viral nucleotide acids. In addition, pathological observation, immunohistochemical staining and the Western blotting were used to identify the expression of viral protein 1(VP1). Results: Pathological observation found that after infection, specific pathological changes, such as bleeding, inflammatory cell infiltration, appeared in the myocardium tissue, lung tissue, skeletal muscle, small intestine, brain tissue and other organs of mice. Immunohistochemical staining showed that all five tissues of the infected mice had EV71 virusspecific antigen distribution. Western blotting analysis demonstrated that EV71 VP1 protein was expressed in all five tissues. Conclusion: The onedayold ICR neonatal EV71infected mouse model was successfully established.
收稿日期: 2017-04-21
基金资助:
国家自然科学基金资助项目(81601751)；镇江市社会发展项目(SH2015040)
通讯作者:
陈建国(通讯作者)，主任技师，硕士生导师，E-mai: cjg02@126.com
作者简介: 秦闫燕(1991—)，女，硕士研究生
引用本文:
秦闫燕1， 卢洁1， 李季霖1， 刘海冰2， 茅凌翔2， 邵启祥1， 陈建国2. 肠道病毒71型ICR乳鼠感染模型的建立[J]. 江苏大学学报:医学版, 2017, 27(5): 385-390.
QIN Yan-yan1, LU Jie1, LI Ji-lin1, LIU Hai-bing2, MAO Ling-xiang2, SHAO Qi-xiang1, CHEN Jian-guo2. Establishment of enterovirus 71-infected ICR neonatal mice model. Journal of Jiangsu University(Medicine Edition), 2017, 27(5): 385-390.
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2017, Vol. 27 
