STAT3 Y705与S727磷酸化水平的相互调控

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摘要目的: 明确信号传导与转录激活因子3(signal transducer and activator of transcription 3，STAT3)中Y705与S727在磷酸化过程中的相互作用。方法: 通过构建STAT3 Y705F和S727A两个磷酸化位点突变的质粒，将其分别转染至TSC2-/-MEF细胞中，蛋白质印迹法检测STAT3 Y705F对STAT3 S727磷酸化水平和STAT3 S727A对STAT3 Y705磷酸化水平的影响。结果：基因测序结果显示编码丝氨酸的TCC突变为GCC，编码酪氨酸的TAC突变为TTC；蛋白质印迹检测结果显示转染STAT3 Y705F组中STAT3 Y705的磷酸化水平显著低于转染HASTAT3组，且STAT3 Y705F下调STAT3 S727磷酸化水平；转染STAT3 S727A组中STAT3 S727的磷酸化水平显著低于转染HASTAT3组，且STAT3 S727A下调Y705磷酸化水平。结论： STAT3中Y705与S727两个磷酸化位点具有协同作用。

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关键词 ：信号传导与转录激活因子3, STAT3 Y705, S727, 突变, 磷酸化

Abstract：Objective: To clarify the interactions between phosphorylated Y705 and phosphorylated S727 in signal transducer and activator of transcription 3(STAT3). Methods: Mutant plasmids of STAT3 Y705F and S727A were constructed and transfected into TSC2-/- MEF cell line, respectively. The phosphorylation level of S727 in STAT3 Y705F, and the phosphorylation level of Y705 in STAT3 S727A were detected by Western blotting, respectively. Results: Gene sequencing showed that TCC were mutated into GCC and TAC were mutated into TTC. Western blotting demonstrated that Y705 and S727 have been mutated successfully and the Y705F mutation downregulated phosphorylation level of S727 in STAT3, while S727A mutation downregulated phosphorylation level of Y705 in STAT3. Conclusion: There is a synergistic effect between phosphorylated Y705 and phosphorylated S727 in STAT3.

收稿日期: 2017-04-21

基金资助:

国家自然科学基金资助项目(81273202，81671541)；江苏省大学生创新训练计划(201610299020Z)；江苏省临床医学科技专项（BL2013024）

通讯作者: 邵启祥(通讯作者)，教授，博士生导师，E-mail：shao_qx@ujs.edu.cn

作者简介: 刘月芳(1990—)，女，硕士研究生；王珂(1996—)，共同第一作者，女，本科生；丁璟(1995—)，共同第一作者，女，本科生；

引用本文:

刘月芳，王珂，丁璟，王荟，吴皓杰，王小铃，王娟，夏圣，周小明，邵启祥. STAT3 Y705与S727磷酸化水平的相互调控[J]. 江苏大学学报:医学版, 2017, 27(04): 327-331. LIU Yue-fang, WANG Ke, DING Jing, WANG Hui, WU Hao-jie, WANG Xiao-ling, WANG Juan, XIA Sheng, ZHOU Xiao-ming, SHAO Qi-xiang. Regulate each other of phosphorylation level of STAT3 Y705 and S727. Journal of Jiangsu University(Medicine Edition), 2017, 27(04): 327-331.

链接本文:

http://zzs.ujs.edu.cn/xbyxb/CN/ 或 http://zzs.ujs.edu.cn/xbyxb/CN/Y2017/V27/I04/327

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