Abstract：Objective: To explore the effect of cyclophilin A on the autophagy activation in RAW264.7 cells, one of the murine microphage line. Methods: RAW264.7 cells were incubated with various concentrations of cyclophilin A(0,1,10,100,500,1000 ng/mL) to figure out the optimal nontoxic concentrations of cyclophilin A for subsequent test. Western blotting was used to detect the expression of autophagy-related proteins LC3 Ⅰ and LC3Ⅱ. The RAW264.7 cells were treated with 100 ng/mL cyclophilin A for 6 hours, and then number of autophagosome was observed by fluorescence microscopy and transmission electron microscopy. Results: With the increasing of cyclophilin A concentration autophagy was gradually induced and reached the peak at the concentration of 100 ng/mL. Compared with control group, the number of autophagosomes significantly increased according to fluorescence and transmission electron microscopic observation. Conclusion: Cyclophilin A could induce autophagy in RAW264.7 macrophages.
［Key words］cyclophilin A; autophagy; microphages; atherosclerosis
李静, 薛志强, 成扶雨, 严金川, 王中群, 袁伟. 亲环素A对RAW264.7巨噬细胞自噬的影响[J]. 江苏大学学报:医学版, 2017, 27(03): 229-.
LI Jing, Xue-Zhi-Qiang, Cheng-Fu-Yu, Yan-Jin-Chuan, Wang-Zhong-Qun, Yuan-Wei. Effect of cyclophilin A on the autophagy induction in the murine macrophage line RAW264.7. Journal of Jiangsu University(Medicine Edition), 2017, 27(03): 229-.
2017, Vol. 27 
