Sequence and phylogenetic analysis of an enterovirus 71 strain isolated in Nanjing
ZHOU-Jin1， CAO Xi-hua2， CAO Tong1， CHEN Hong-bing1
(1. Department of Clinical Laboratory, Children′s Hospital Affiliated to Nanjing Medical University, Nanjing Jiangsu 210008; 2. Department of Anesthesiology, the First Affiliated Hospital of Nanyang Medical College，Nanyang Henan 473058,China)
Abstract：Objective: To sequence and analyze the complete genome of an enterovirus 71 isolate(EV71-NJ2012) isolated in Nanjing(2012),and to compare the sequences deposited in GenBank. Methods: According to the genome information from GenBank,8 pairs of primer were designed to cover the whole genome. The genome sequences were determined by RT-PCR sequencing method, and the sequences were analyzed using bioinformatic software (MEGA 4.0 and DNAStar). Results: The full genomic length of EV71-NJ2012 was 7 404 bp，which contained 5′UTR (742 bp)，the open reading frame (ORF, 6 582 bp) and 3′UTR (82 bp).The ORF encoded a polyprotein with 2 193 amino acid residues. The viral sequences belong to the sub-type C4a genotype and had the maximum homology with Shanghai isolate,however,its sequence was quite different with the Taiwan and Hubei isolates. Conclusion: The genome structure of EV71-NJ2012 strain is consistent with enterovirus.The phylogenetic relationships of the EV71-NJ2012 strain with other EV71 strains are different.EV71-NJ2012 strain has the maximum sequence homology with Shanghai isolate, and is quite different with the Taiwan and Hubei isolates.It maybe infer that EV71-NJ2012 and Shanghai strains probably originate from the same EV71 strain.
收稿日期: 2014-04-29
通讯作者:
陈红兵（通讯作者），E-mail：115468172@qq.com
作者简介: 周晋（1985—），男，江苏南京人，技师，主要研究方向为病原学；
引用本文:
周晋1， 曹喜华2， 曹彤1， 陈红兵1. 1株肠道病毒71型南京分离株的全基因组序列的测定及遗传进化分析[J]. 江苏大学学报:医学版, 2014, 24(04): 312-315.
ZHOU-Jin，CAO Xi-hua，CAO Tong，CHEN Hong-bing. Sequence and phylogenetic analysis of an enterovirus 71 strain isolated in Nanjing. Journal of Jiangsu University(Medicine Edition), 2014, 24(04): 312-315.
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2014, Vol. 24 
