Abstract：Objective: To observe the effect of CO-releasing molecules-2（CORM-2） on the random narrow pedicle skin flap in the SD rats with ischemia-reperfusion injury(IRI)，and discuss the possible mechanism. Methods: Established IRI models of random narrow pedicle skin flap in rats.A total of 36 SD rats were divided into two groups at random：CORM-2 group，models of random narrow pedicle skin flap and treated by CORM-2；NS group，models of random narrow pedicle skin flap and treated by normal saline(NS).Test the activity of NF-κ B by Western blotting. The content of malonaldehyde(MDA) in tissue of skin flap was detected by phenobarbitone acid；the activity of superoxide dismutase(SOD) in tissue of skin flap was detected by xanthine oxidase；the level of IL-6 and tumor necrosis factor-α(TNF-α) in blood serum was detected by ELISA；pathological changes were observed with light microscope in the 3rd, 5th, 7th day after operation；survival rate of skin flap was the average survival length of skin flap deciding practical length of skin flap. Results: ① The skin flap uncovered by the 3rd, 5th, 7th day，compared with NS group，the content of MDA in the skin flap of CORM-2 group lower(P<0.01)，the activity of SOD significantly increased (P<0.01). ② The skin flap uncovered by 3rd, 5th, 7th day，compared with NS group，the level of IL-6 and TNF-α in blood serum lower(P<0.01). ③ Histopathology showed that abundant capillary were noticed by application of CORM-2. ④ CORM-2 can effectively restrain the NF-κ B activation in random narrow pedicle skin flap model. ⑤ The skin flap was uncovered by the 7th day，compared with NS group，survival rate of skin flap of CORM2 group significantly higher(P<0.01). Conclusion: CORM-2 effectively reduced the narrow pedicle skin flap of rats with IRI, and improved the survival rate of flap.
高亮， 邰宁正. 外源性一氧化碳释放分子对大鼠随意窄蒂皮瓣缺血再灌注损伤的保护作用[J]. 江苏大学学报:医学版, 2014, 24(04): 307-311.
GAO Liang, TAI Ning-zheng.
Protection of CO-releasing molecules-2 against the random narrow pedicleskin flap with ischemia-reperfusion injury in rats. Journal of Jiangsu University(Medicine Edition), 2014, 24(04): 307-311.
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2014, Vol. 24 
