Abstract：Objective: To identify the expression of samll noncoding RNA(snRNA) T64 detected by transcriptome sequencing in Salmonella enterica serovar Typhi(S.Typhi) and explore its functions preliminarily. Methods: The native expression of snRNA T64 in S.Typhi was confirmed by reverse transcription PCR (RTPCR) and PCR with specific primers. Overexpression strain of snRNA was constructed by transforming a recombinant plasmid containing sequence of snRNA T64 to the wild type strain of S. Typhi. Microarray was used to analyse the gene expression difference between overexpression strain and control strain in logarithmic growth phase. Some microarray results were confirmed by quantitative real-time PCR(qRT-PCR). Results: The expression of snRNA T64 was confirmed by the RT-PCR. The results of microarray assay revealed that there were 20 genes expression upregulated in the overexpression strain of snRNA T64, while 7 genes expression downregulated, compared with the control strain in logarithmic growth phase. qRT-PCR results of expressions of 4 selected genes matched the microarray results. Conclusion: There were expression of snRNA T64 in S.Typhi, which might play important roles in gene expression regulation.
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2014, Vol. 24 
