Abstract：Objective: To study the expression of Tolllike receptor 2（TLR2）,Tolllike receptor 4（TLR4） on doxorubicininjured cardiac cells in newborn rats，and to discuss the possible mechanism on doxorubicininjured cardiac cells. Methods: Doxorubicin was administered to establish the injury model of primary cultured cardiomyocytes in newborn rats. Primary cultured cardiomyocytes were randomly divided into two groups: control group：myocardial cell suspension continued cultivate with serumfree medium and no drugs put into; DOX group: with doxorubicin 1 mg/L in cultured fluid.The expression of TLR2 and TLR4 protein in cardiac cells was checked with immunohistochemical after they were operated for 6 hours. The semiquantitative analysis of TLR2 and TLR4 protein was checked with Western blot after they were operated for 2 hours, 6 hours respectively. Results: The expression of TLR2 and TLR4 on the cardiac cells in DOX group are markedly increased when compared with control group(P<0.01). Conclusion: TLR2 and TLR4 may mediate and take part in the innate immune response of doxorubicininjured cardiac cells as a pattern recognition,and this was one of the cardiac cells injuring mechanism.
收稿日期: 2012-07-18
作者简介: 束进（1971—），男，江苏镇江人，副主任医师，主要从事儿童心血管疾病的研究工作。
引用本文:
束进， 刘欣， 王海波， 彭俊娟， 朱红英. 多柔比星对乳鼠心肌细胞TLR2、TLR4表达的影响[J]. 江苏大学学报:医学版, 2012, 22(5): 391-393.
SHU Jin, LIU Xin, WANG Hai-bo. Expression of TLR2，TLR4 on doxorubicininjured cardiac cells in newborn rats. Journal of Jiangsu University(Medicine Edition), 2012, 22(5): 391-393.
2012, Vol. 22 
