Effect of OsmY on gene expressional regulation of Salmonella enterica serovar Typhi at early stage of hyperosmotic stress
1.School of Medical Science and Laboratory Medicine, Jiangsu University, Zhenjiang Jiangsu 212013; 2.Clinical Laboratory, Second Affiliated Hospital of Soochow University, Suzhou Jiangsu 215004, China
Abstract：Objective: To explore the effect of OsmY on gene expressional regulation of Salmonella entericaserovar Typhi (S.Typhi) at the early stage of hyperosmotic stress.Methods: The osmY gene deleted mutant of S.Typhi was generated through homologous recombination mediated by a suicide plasmid; the gene expression profiles of the wild type strain and the osmY deleted mutant at early stage of hyperosmotic stress was investigated by genomic microarray assay; qRTPCR was performed to validate the results of microarray assay. Results: The osmY deleted mutant of S.Typhi was prepared successfully; analysis of genomic microarray assay showed that 27 genes were up regulated and 128 genes were down regulated in the osmY deleted mutant at the early stage of hyperosmotic stress compared to the wild type strain. The results of qRTPCR assay were consistent with the results of microarray assay. Conclusion: osmY of S.Typhi may be a regulator played an important role in regulating gene expression at early stage of hyperosmotic stress.
2012, Vol. 22 
