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| Fluorescence bioimaging and colorimetric detection of
H2O2 in tumor cells based on Fe-CDs nanozymes
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| LI Xiaoran, WU Chunyan, LI Feng, PANG Zhonghao, XU Xiuquan |
| 1. Affiliated Hospital of Jiangsu University, Zhenjiang, Jiangsu 212001, China; 2. College of Traditional Chinese Medicine, Bozhou University, Bozhou, Anhui 236800, China |
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Abstract The novel Fe doped carbon dots (FeCDs) with good photoluminescent properties were rapidly synthesized by microwaveassisted method with ferric citrate and polyethyleneimine as raw materials. The results show that the FeCDs have good stability under the conditions of broad pH range from 3 to 9, high ion strengths and long storage time. The prepared FeCDs can easily penetrate and accumulate into the cell, which can be used as effective probe for biological imaging of A549 cells. The FeCDs display distinct peroxidaselike (POD) activities, which is evidenced by the catalyzed oxidation of 3,3′,5,5′-tetramethylbenzidine (TMB) into blue oxidized products in the presence of H2O2. A colorimetric method for the determination of H2O2 is established based on the results. Under optimal reaction conditions, the colorimetric method for H2O2 detection is constructed with good linear relationship from 0 to100.00 μmol/L and detection limit down to 0.13 μmol/L. The highly selective and ultrasensitive method is successfully applied to detect H2O2 inside A549 tumor cells with good recoveries of 98.4%-102.8%, which can verify the proposed method.
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| Fund:江苏大学附属医院高级人才计划（JDFYRC201700）； 江苏大学附属医院“北固英才”项目 (2022); 安徽省高等学校科学研究重大项目 (2023AH040316)
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