Abstract：With the degradable polylactic acid（PLA） as matrix, the porous membrane with threedimensional reticular structure was prepared by highvoltage electrostatic spinning technology. Using different concentration Bletilla striata as impregnation solution, Bletilla striata polysaccharide hemostatic composite membrane with polylactic acid (PLA) as nanoskeleton was prepared by ultrasonic and hot pressing. The composite membrane was characterized by scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR), universal tensile testing machine and thermogravimetric analyzer (TGA). The hemostatic properties and healing properties of tissue wounds were tested. The results show that the Bletilla striata polysaccharide are fully filled in the threedimensional pores of the composite membrane. The tensile strength of the composite membrane is up to 9.512 MPa, which can be stably existed below 80 ℃. Compared with the saline control group, the composite membrane has good hemostatic function and obvious hemostasis effect, and the hemostasis time is reduced by 60%.
