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Journal of Jiangsu University(Medicine Edition)
 
2022 Vol.32 Issue.03
Published 2022-05-25
0
2022 Vol. 32 (03): 0- [Abstract] ( 35 ) [HTML 1KB] [ PDF 316KB] ( 421 )
185 Screening anti-pituitary adenoma drugs from global marketed drug data based on artificial intelligence and validating with in vitro experiments
LYU Tingting, ZHANG Zirui, LI Shang,et al.
Objective: Exploring potential treatment drugs for pituitary adenoma from marketed drugs based on artificial intelligence technology and drug relocation strategies. Methods: The gene expression profile of pituitary adenoma was analyzed by Gene Expression Omnibus （GEO） database, and the highest scoring Hub gene was screened out. The drug repositioning strategy was used to find therapeutic drugs targeting Hub gene from the global market drug database, and the interaction between drug and target was validated by artificial intelligence technology. In cell experiments, the effect of tamoxifen on the proliferation ability of mouse pituitary adenoma AtT20 cells and rat pituitary adenoma GH3 cells was detected by CCK8 method, the apoptosis rate was detected by flow cytometry, and the expressions of key proteins in the phosphatidylinositol-3-kinase （PI3K）/protein kinase B （AKT） apoptosis pathway was detected by Western blotting. Results: A total of 96 differentially expressed genes were identified from macroadenomas and microadenomas, among which signal transducer and activator of transcription 6 （STAT6） was the gene with the highest score. Tamoxifen was found to be the therapeutic drug targeting STAT6 by relocating from the database. The interaction between tamoxifen and STAT6 was confirmed by artificial intelligence and molecular docking. Finally, in vitro experiments showed that tamoxifen could inhibit the proliferation of pituitary adenoma cells and hormone secretion. Conclusion: Tamoxifen can inhibit the growth of pituitary adenoma, providing strong evidence for the target research and precise treatment of pituitary adenoma.
［Key words］pituitary adenoma; artificial intelligence; drug repositioning; STAT6; Tamoxifen
2022 Vol. 32 (03): 185-193 [Abstract] ( 51 ) [HTML 1KB] [ PDF 17589KB] ( 664 )
194 miR-216a and miR-301a promote the proliferation of pulmonary arterial smooth muscle cells under hypoxia via regulating BMPR2
Objective: To investigate the effects of miR-216a and miR-301a on the proliferation of human pulmonary arterial smooth muscle cells （HPASMC） under hypoxia and to explore the potential mechanisms. Methods: The proliferation activity of HPASMC under normoxia and different hypoxia time was detected by CCK8 assay, and qRT-PCR was used to detect the expression of miR-216a, miR-301a and bone morphogenetic protein receptor type 2 （BMPR2） mRNA in each group. The proliferation ability and proliferating cell nuclear antigen （PCNA） expression levels of HPASMC transfected with anti-miR-216a or anti-miR-301a mimics were detected by CCK8 assay and Western blotting, respectively. The relationship between BMPR2 and miR216a, BMPR2 and miR301a were detected by dual luciferase reporter assay. CCK8 assay and Western blot were used to detect the proliferation ability of HPASMC and the expression levels of BMPR2 and PCNA after simultaneous transfection of miR-216a or miR-301a combined with BMPR2 overexpression plasmid, respectively. Results: With the prolongation of hypoxia time, the proliferation activity of HPASMC and the expression of miR-216a increased, while the expression of BMPR2 mRNA gradually decreased. The expression level of miR-301a was the highest in HPASMC exposed to hypoxia for 24 hours. Down-regulation of miR-216a and miR-301a significantly inhibited the proliferation activity and PCNA expression of HPASMC. Dual luciferase reporter assay confirmed that BMPR2 was a target of miR-216a and miR-301a, respectively. Simultaneously transfected with miR-216a or miR-301a combined with BMPR2 overexpression plasmid, the expression of BMPR2 decreased, and the cell proliferation activity and PCNA expression increased. Conclusion: miR-216a and miR-301a induce pulmonary arterial hypertension by promoting the proliferation of HPASMC under hypoxia via inhibiting the expression of BMPR2.
［Key words］miR-216a; miR-301a; pulmonary arterial hypertension; pulmonary arterial smooth muscle cells; bone morphogenetic protein receptor type 2
2022 Vol. 32 (03): 194-199,218 [Abstract] ( 35 ) [HTML 1KB] [ PDF 5019KB] ( 508 )
200
2022 Vol. 32 (03): 200-206 [Abstract] ( 41 ) [HTML 1KB] [ PDF 6791KB] ( 621 )
207
2022 Vol. 32 (03): 207-212 [Abstract] ( 34 ) [HTML 1KB] [ PDF 4253KB] ( 558 )
213 Effects of Nrg1 gene knockdown in prefrontal cortex on formation of schizophrenia-like phenotypes in mice
CONG Qijie, ZENG Rongsen, LIU Yunxi, et al.
Objective: To explore the effect of neuregulin 1 （Nrg1） knockdown in prefrontal cortex （PFC） on the formation of schizophrenia-like phenotypes in mice and its potential mechanism. Methods: A total of 45 adult male C57BL/6 mice were divided into 3 groups with 15 mice in each group based on open field test, including control group, negative control group, Nrg1-siRNA group. Stereotaxic surgery was performed to inject 2 μL negative control siRNA and Nrg1-siRNA in mice PFC of negative control group and Nrg1-siRNA group, while control group were injected with 2 μL PBS. Nrg1 mRNA and NRG1 protein expression in prefrontal cortex were detected by qRT-PCR and Western blotting. The mice with Nrg1 gene knockdown in PFC were evaluated on the prepulse inhibition, open field test, and elevated plus maze. Western blotting was used to detect the expression of vesicular GABA transporter （VGAT） and vesicular glutamate transporter 1 （VGLUT1）. Results: The expression of Nrg1 mRNA and NRG1 protein in the PFC in Nrg1-siRNA group were significantly decreased compared with control group （P<0.01）. In behavior tests, compared with control group, Nrg1-siRNA group showed lower prepulse inhibition（P<0.05）, higher distance and speed of spontaneous movement（both P<0.01）, and the percentage of times and time to enter the open arm was significantly reduced（both P<0.01）. The ratio of VGLUT1/VGAT expression in Nrg1-siRNA group was significantly increased compared with control group. Conclusion: Nrg1 gene knockdown in mice PFC could lead to significant schizophrenia-like behavior characterized by prepulse inhibition disorder, increased spontaneous movement and anxiety, which may be related to the imbalance between inhibitory and excitatory neurotransmitter transporters.
［Key words］neuregulin 1; schizophrenia; mice; prefrontal cortex; brain stereotactic injection
2022 Vol. 32 (03): 213-218 [Abstract] ( 46 ) [HTML 1KB] [ PDF 4260KB] ( 1362 )
219 Inhibitory effect of curcumin on human carcinoma SMMC-7721 cells xenografted in nude mice and its mechanism
SUN Jun, XU Wei, LU Rongzhu,et al.
Objective: To investigate the inhibitory effect of curcumin on the growth of human carcinoma SMMC-7721 cell transplanted tumor in nude mice and its potential mechanism. Methods: Transplanted tumor model of SMMC-7721 cells was established in 20 nude mice, and the tumorbearing nude mice were equally divided into control group and three different concentrations of curcumin （10, 50 and 100 mg/kg） groups, with 5 mice in each group. The mice in each group was intraperitoneally injected with normal saline or corresponding dose of curcumin, once a day, 200 μL each time, for 16 consecutive days. The body weight and tumor volume of each group were observed and recorded every other day from the day of administration. After 16 days, tumor tissues were harvested and tumor inhibition rates of curcumin treatment groups were calculated by the weighed tumor body. The expression levels of ER stress-related proteins and apoptotic proteins in tumor tissues were analyzed by Western blotting. The positive expressions of glucose requlated protein 78 （GRP78） and inositol requiring enzyme 1 （IRE1） were analyzed by immunohistochemical staining. Results: Compared with that in the control group, there was no significant difference in the weight gain of nude mice in different doses of curcumin groups （P>0.05）, whereas the volume of transplanted tumor in 50 and 100 mg/kg curcumin groups was significantly smaller than that in the control group and 10 mg/kg curcumin group（P<0.05）. The tumor inhibition rates of 10, 50 and 100 mg/kgcurcumin groups were 35.3%, 45.7% and 54.7%, respectively. Western blotting showed that GRP78, IRE1, C/EBP homologous protein （CHOP） and Cleaved caspase-3 protein expressions in 50 and 100 mg/kg curcumin groups were significantly up-regulated compared with that in the control group, and the protein expressions of p-PERK, p-eIF2α and Bcl-2 in 10, 50 and 100 mg/kg curcumin groups were higher than those in the control group （both P<0.05）. Results of immunohistochemical staining showed that the positive expression of proteins GRP78 and IRE1 in tumor tissues of all curcumin groups were markedly increased compared with the control group（all P<0.05）. Conclusion: Curcumin could inhibit the growth of subcutaneous transplanted tumor in nude mice with human hepatoma SMMC-7721 cells, which may be attributed to activating endoplasmic reticulum stress.
［Key words］curcumin; hepatocellular carcinoma cell; endoplasmic reticulum stress; unfolded protein reaction; apoptosis
2022 Vol. 32 (03): 219-225，230 [Abstract] ( 40 ) [HTML 1KB] [ PDF 13987KB] ( 383 )
226 Leucine-rich repeat sequence protein 1 promotes breast cancer cell migration and invasion by upregulating the expression of#br# mammalian rapamycin target proteins
WANG Le, ZHU Xiaoren, PENG Shiqing，et al .
Objective: To investigate the expression of leucinerich repeat protein 1 （LRR1） in breast cancer cells and its effect on cells biological function. Methods: Human breast cancer MDA-MB-231, HCC70 cell lines and normal breast epithelial MCF-10A cells were cultured; Western blotting was used to detect the expression of LRR1. Human breast cancer MDA-MB-231 and HCC70 cell lines were divided into interference control group, LRR1 interference group, LRR1 overexpression group and empty vector control group, which were transfected with LRR1 control, interference, overexpression and empty vector sequences, respectively; Transwell assay was used to detect migration and invasion ability of breast cancer cells; Western blotting was used to detect the expression of mammalian target of rapamycin （mTOR）. Results: Compared with normal human breast epithelial MCF-10A cells, the expression level of LRR1 in breast cancer MDF-MB-231 and HCC70 cells was significantly increased （both P<0.01）. In human breast cancer MDA-MB-231, HCC70 cell line, compared with the interference control group, LRR1 interference group significantly decreased the number of cell migration and invasion （all P<0.01）, and the expression level of m-TOR was significantly decreased（both P<0.01）; compared with the empty vector control group, LRR1 overexpression group significantly increased the numbers of migration and invasion（both P<0.01）, and the expression level of m-TOR protein was significantly increased （both P<0.01）. Conclusion: LRR1 is highly expressed in breast cancer cell lines and promotes their migration and invasion.
［Key words］breast cancer; leucinerich repeat protein 1; mammalian target of rapamycin（m-TOR）; cell migration; cell invasion
2022 Vol. 32 (03): 226-230 [Abstract] ( 34 ) [HTML 1KB] [ PDF 12633KB] ( 435 )
231 concentrations of fetal bovine serum on proliferation and differentiation of primary cadiac fibroblasts in vitro
LI Shanshan, DANG Shipeng, ZHANG Zhenye, et al
Objective: To investigate the effects of different concentrations of fetal bovine serum on proliferation and differentiation of primary cardiac fibroblasts in vitro. Methods: Cardiac fibroblasts were isolated from SD neonatal rats by enzyme digestion and differential adhesion method, then cultured with different concentrations of fetal bovine serum （1%,5%,10% and 20%）. The cell morphology was observed, and the expression of α-SMA protein was detected by immunofluorescence confocal assay. The migration ability of cardiac fibroblasts was detected by scratch assay. The proliferation ability of cardiac fibroblasts was detected by CCK8 assay. Results: With the increase of fetal bovine serum concentration, the cell morphology gradually became round, the cell surface area increased, the proportion of αSMA positive cells increased, the differentiation of cardiac fibroblasts to myofibroblasts increased, and the cell migration ability increased. The proliferation ability of myocardial fibroblasts was the most vigorous when cultured with the highest concentration of fetal bovine serum （20%）. The proliferation ability of myocardial fibroblasts was the lowest when cultured with low concentration of fetal bovine serum （1%）. In 5% and 10% concentration of serum treated cardiac fibroblasts, the proliferation rate of cells was between high and low concentration of fetal bovine serum. Conclusion: Cardiac fibroblasts cultured in high fetal bovine serum concentration can lead to differentiation of myocardial fibroblasts. Compared with 10% and 20% concentration of serum, cardiac fibroblasts cultured in 5% serum concentration have less differentiation. Compared with 1% concentration of serum, cardiac fibroblasts cultured in 5% serum concentration have better proliferation ability. It is better to select 5% serum concentration to culture cardiac fibroblasts.
［Key words］cardiac fibroblasts; in vitro culture; concentrations of fetal bovine serum; α-SMA; differentiation
2022 Vol. 32 (03): 231-235 [Abstract] ( 36 ) [HTML 1KB] [ PDF 7135KB] ( 759 )
236 Effects of febuxostat on Treg/Th17 balance and vascular endothelialfunction in rats with hyperuricemia
CHEN Ning, SUN Hong, ZHENG Weiping1, et al.
Objective: To investigate the effects of febuxostat on Treg/Th17 balance and vascular endothelial function in hyperuricemia rats. Methods: Thirtytwo male SD rats were randomly divided into 4 groups with 8 rats in each group, including normal control group, model group, febuxostat group and allopurinol group. Rats in model group and experimental group were given potassium oxazinate and yeast extract by gavage to establish a rat model of hyperuricemia. Febuxostat （10 mg·kg-1·d-1） and allopurinol （50 mg·kg-1·d-1） were given by gavage respectively to febuxostat group and allopurinol group for 6 weeks. After drug treatment, the levels of serum uric acid, interleukin-6 （IL-6）, nitric oxide （NO）, and endothelin-1 （ET-1） were measured by ELISA. The changes of Tregs and Th17 cells were measured by flow cytometry. HE and Masson staining were used to observe pathological changes of aortic endothelium tissues. Results: After febuxostat treatment, the levels of serum uric acid, IL-6 were significantly decreased （P<0.05）. The effects of febuxostat on reducing serum uric acid, IL-6 was better than that of allopurinol （P<0.05）. The levels of serum NO in the febuxostat group were higher than that in the model group （P<0.05）, while the levels of ET-1 were lower than that in the model group （P<0.05）. After febuxostat treatment, the percentages of Th17 cells were decreased while the ratios of Treg/Th17 were increased （P<0.05）. Histopathologic results showed that febuxostat could improve the swelling of endothelial cells and reduce the proliferation of fibrous tissue and deposition of collagen fibers. Conclusion: Febuxostat can improve vascular endothelial dysfunction in hyperuricemia rats, which may be related to its correction of Treg/Th17 imbalance.

［Key words］Febuxostat; hyperuricemia; Treg/Th17 balance; interleukin-6; nitric oxide; endothelin-1
2022 Vol. 32 (03): 236-240 [Abstract] ( 46 ) [HTML 1KB] [ PDF 5703KB] ( 521 )
241 Effect of total flavonoids of Scutellaria baicalensis Georgi on Camk2d and Ppp3r2 expression in cartilage of knee osteoarthritis rats
LIU Dakai, ZHENG Xifu, LI Anshi
Objective: To investigate the effect of total flavonoids of Scutellaria baicalensis Georgi （TFSB） on calcium/calmodulin dependent protein kinaseⅡdelta（Camk2d） and protein phosphatase 3 regulates subunit 2（Ppp3r2） expression in cartilage of knee osteoarthritis rats. Methods: The knee osteoarthritis model was prepared by injecting papain into the knee joint cavity. The rats in the negative control group and the model group were given normal saline by intragastric administration. Rats in TFSB low-dose group, middle-dose group and high-dose group were given the TFSB by gavage at the doses of 12.5, 25, and 50 mg/kg. The positive control group rats were gavaged with celecoxib （10 mg/kg）, and treated for 14 consecutive days. The pathological knees joint of rats was observed and Makin′s score were performed, and the TNF-α, IL-1β, IL-6, Camk2d and Ppp3r2 of knee cartilage tissue were detected by ELISA. The levels of Camk2d and Ppp3r2 protein in knee cartilage tissue were detected by Western Blotting. Results: In the model group, the knee cartilage fiber connective tissue was proliferated, and the structure was unclear, and the inflammatory cells infiltrated. The low-dose group of TFSB had hyperplasia of articular cartilage fiber connective tissue, irregular cell arrangement and inflammatory cell infiltration, but it was significantly improved compared with the model group. With the increasing of the dose of TFSB, the improvement of the lesion was more obvious. Compared with the negative control group, the Makin′s score, TNF-α, IL-1β, IL6 and Camk2d levels in the knee cartilage tissues of the model group increased, while the Ppp3r2 level decreased（all P<0.05）. After the TFSB or celecoxib treatment, compared with the model group, the Makin′s score, TNF-α, IL-1β, IL-6 and Camk2d levels in each dose group of TFSB and the positive control group decreased, and the level of Ppp3r2 increased, and the effect of each dose group of TFSB was dosedependent（all P<0.05）. The indicators between the high-dose group of TFSB and the positive control group were no significant difference（all P>0.05）. Conclusion: TFSB may exert therapeutic effect in rats with knee osteoarthritis by increasing the expression of Ppp3r2 and reducing the expression of Camk2d in cartilage.
［Key words］total flavonoids of Scutellaria baicalensis Georgi; knee osteoarthritis; calcium/calmodulin dependent protein kinase Ⅱdelta; protein phosphatase 3 regulates subunit 2; rats; cartilage
2022 Vol. 32 (03): 241-245，250 [Abstract] ( 43 ) [HTML 1KB] [ PDF 10594KB] ( 1493 )
246 Inhibitory effect of gossypol acetate on the growth of  glioma cells via induction of ferroptosis
YANG Yang, YANG Mengting, XU Xiao
Objective: To investigate the effect of gossypol acetate （GAA） on ferroptosis in human glioma cells. Methods: Human brain glioma U87MG, LN229 and U251MG cells in logarithmic growth phase were selected and divided into two groups, treated with 0 or 10 μmol/L GAA, respectively, CCK-8 and clony formation assays were used to detect cell proliferation abilities. QRT-PCR and Western blotting were used to detect the expression of glutathione peroxidase 4（GPX4） in mRNA and protein levels, respectively. Also, the contents of reduced glutathione （GSH） and malondialdehyde （MDA） were detected by chemiluminescence. In addition, three kinds of human glioma cells were co-cultured with 0 or 10 μmol/L GAA combined with 0, 1 μmol/L Ferrostatin-1, respectively, and the cell proliferation rate was detected by CCK-8 method. Results: Compared with 0 μmol/L GAA group, the relative proliferation rate and clonal formation ability of U87MG, LN229 and U251MG cells in GAA 10 μmol/L group were significantly decreased （all P<0.05）, the expression levels of GPX4 mRNA and protein in LN229 and U251MG cells decreased significantly （all P<0.05）, the content of GSH decreased significantly and the content of lipid peroxidation product, malondialdehyde increased remarkably（P<0.05）, while the above indexes in U87MG cells did not change greatly （P>0.05）. Compared with 10 μmol/L GAA+0 μmol/L Ferrostain-1 group, the relative proliferation rate of three brain glioma cells in 10 μmol/L GAA+1 μmol/L Ferrostatin-1 group was significantly increased（all P<0.05）. Conclusion: Gossypol acetate promotes ferroptosis in glioma cells, and LN229 and U251MG cells are more sensitive than U87MG cells.
［Key words］glioma; gossypol acetate; ferroptosis;Ferrostatin-1; glutathione peroxidase 4（GPX4）
2022 Vol. 32 (03): 246-250 [Abstract] ( 32 ) [HTML 1KB] [ PDF 10276KB] ( 415 )
251 Analysis of prognostic factors in elderly breast cancer patients  based on SEER database
CHENG Meilian, SUN Wen, LI Xiaoqin, et al.
Objective: To analyze the prognostic factors of breast cancer-specific and non-breast cancer-specific deaths in elderly female breast cancer patients, using a competing risk model based on the SEER database. Methods: Female breast cancer patients aged ≥70 years were selected from 2006 to 2010 in the SEER database, and the “cmprsk” package in the R statistical software was used to perform univariate and multivariate analysis of the competing risk model. Results: A total of 31 823 elderly patients were included in the study, and 13 075 died during follow-up. Among them, 3 735 died from breast cancer, and 9 340 died from non-breast cancer causes. The 1-, 3-, and 5-year breast cancer-specific mortality rates were 1.47%, 5.39% and 8.71%, respectively; and the non-breast cancer mortality rates were 2.17%, 8.49% and 15.79%, respectively. The results of multivariate analysis showed that advanced age, single status, black race, estrogen receptor positivity, poorly differentiated, advanced T and N stages and no radiotherapy were independent poor prognostic factors affecting the cause of breast cancer death in elderly patients; advanced age, single status, estrogen receptor positivity, advanced T stage and other factors were independent poor prognostic factors affecting non-breast cancer death in elderly patients. Conclusion: Elderly breast cancer patients are more likely to die from non-breast cancer than from breast cancer, and their radiotherapy could reduce breast cancer-specific and non-specific breast cancer deaths.
［Key words］elderly; breast cancer; SEER database; estrogen receptor; competing risk model; prognostic factors
2022 Vol. 32 (03): 251-255 [Abstract] ( 45 ) [HTML 1KB] [ PDF 3057KB] ( 751 )
256 Clinical features and early identification of children with chronic food protein-induced enterocolitis syndrome
LONG Yun, QIN Zhen, ZHUO Lin, et al.
Objective: To investigate the clinical features and early identification indexes of children with chronic food protein-induced enterocolitis syndrome （FPIES）. Methods: Thirteen children with chronic FPIES who were admitted to the Department of Gastroenterology, Anhui Children′s Hospital from September 2017 to July 2020, were selected to analyze gender, age at first onset, age at diagnosis, clinical manifestations, laboratory tests, and milk oral tolerance age. In addition, 15 children with acute FPIES during the same period were selected as controls, and the clinical characteristics, laboratory test indexes, endoscopic findings, pathological results and treatment plans between the two groups were compared. Pearson correlation analysis was performed on peripheral blood C-reaction protein （CRP） levels and IL-6 levels in children with chronic FPIES. Results: Among the 13 children with chronic FPIES, there were 7 boys and 6 girls, the median age were 28（2-60） days for onset, 140（57-180） days for diagnosis, and 20（11-32） months for oral tolerance of milk. Meanwhile, among the 15 children with acute FPIES, there were 7 boys, 8 girls, the median age were 90（22-480） days for onset and 230（123-630） days for diagnosis. It is found that diarrhea, bloody stool, fever and poor weight gain are common manifestaion in the chronic FPIES group; in addition, the neutrophil count, CRP and IL-6 were significantly increased, whereas total protein, albumin and hemoglobin were decreased（all P＜0.05）. Based on Pearson correlation analysis, there was a linear correlation between CRP level and IL-6 level（r＝0.90, P＜0.001） in children with chronic FPIES. Conclusion: The chronic FPIES should be considered when presenting the fever, anemia, poor weight gain, as well as the increase of neutrophil count, CRP and IL-6 in patients.
［Key words］food protein-induced enterocolitis; chronic type; milk protein allergy; anemia; oral tolerance
2022 Vol. 32 (03): 256-261 [Abstract] ( 42 ) [HTML 1KB] [ PDF 3887KB] ( 463 )
262 Hemostatic effect and risk factors of delayed bleeding after endoscopic resection of colon polyps
GE Jun, ZHAO Bing, YANG Xi, et al.
Objective: To explore the characterization of endoscopic hemostasis in patients with delayed bleeding after endoscopic resection of colon polyps, and analyze the risk factors of hemostasis failure. Methods: The clinical medical records of 121 patients with delayed bleeding after endoscopic resection of colon polyps who were admitted to Jiangsu Shengze Hospital Affiliated to Nanjing Medical University from January 2013 to April 2021 were retrospectively analyzed, and the clinical data of the patients were collected and sorted. All patients were treated with endoscopic hot biopsy forceps combined with titanium clamps for hemostasis. According to the results of the fecal occult blood （OB）test 1 week after hemostasis, they were divided into successful hemostasis group and failed hemostasis group. Logistic multivariate regression analysis was used to explore the risk factors for the failure of endoscopic hemostasis in patients with delayed hemorrhage after endoscopic resection of colon polyps. Results: Of the 121 patients with delayed bleeding after endoscopic resection of colon polyps in the study, 11 patients（9.09%） had recurrence of blood after surgery, 110 patients（90.91%） had normal hemostasis. The proportion of history of hyperlipidemia, history of hypertension, prolonged prothrombin time, and colon polyp diameter>1.0 cm in the failed hemostasis group was higher than that in the successful hemostasis group （all P<0.05）. Logistic multivariate regression analysis showed that combined with hyperlipidemia, hypertension, prolonged prothrombin time, and colon polyp diameter> 1.0 cm are all risk factors of hemostasis failure in patients with delayed hemorrhage after endoscopic resection of colon polyps （P<0.001）. Conclusion: Delayed bleeding after endoscopic resection of colon polyps has superior hemostasis effect, but there are still some patients with postoperative bleeding. Combined with hyperlipidemia, hypertension, prolonged prothrombin time, colon polyps diameter> 1.0 cm are failure of hemostasis risk factors.
［Key words］colon polyps; endoscopic resection; delayed bleeding;endoscopic hemostasis; hemostasis effect; risk factors
2022 Vol. 32 (03): 262-265 [Abstract] ( 38 ) [HTML 1KB] [ PDF 2623KB] ( 749 )
266 Preparation of sulfate glucosamine liposome and its therapeutic effect on osteoarthritis
JI Shuaishuai, YAN Jie, WANG Ziyin, et al.
Objective: To construct and evaluate glucosamine sulfate liposome （GAS-Lips）, compare the treatment of glucosamine sulfate （GAS） and GAS-Lips on osteoarthritis from anterior cruciate ligament transection （ACLT）. Methods: The GASLips were constructed by film dispersion method and evaluated for their particle size, morphology, drug loading content and retention in vivo. Twentyfour SD rats were randomly divided into sham operation group, ACLT+ untreated group, ACLT+GAS group, and ACLT+GAS-Lips group. In vivo efficacy experiments were carried out by injecting normal saline, GAS and GAS-Lips into the joint cavity, and their therapeutic effects on osteoarthritis were observed. Results: The result of characterization experiment showed that an average particle size is （255.02±3.64） nm, with a spherical shape. The pharmaceutics evaluation showed that the GAS-Lips had a entrapment efficiency of （78.62±1.01）%, and drug loading content of （9.90±0.75）%, and had a good sustained-release effect. In vivo retention experiments showed that the Cy3-Lips group was significantly longer in the joint cavity when compared with the control group （P<0.01）. In vivo efficacy experiments showed that GAS-Lips were more effective in alleviating joint surface damage than free GAS, inhibited the expression of inflammatory factors （both P<0.01）, and had no significant effect on body mass in rats. Conclusion: This study successfully constructed and evaluated a GAS-Lips, improved the retention time and treatment effect of GAS in osteoarthritis, and provided the research basis for its development into osteoarthritis nano-preparation.
［Key words］glucosamine sulfate; liposome; osteoarthritis; joint cavity injection; nanopreparation; inflammatory factors
2022 Vol. 32 (03): 266-271 [Abstract] ( 38 ) [HTML 1KB] [ PDF 13169KB] ( 379 )
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2022 Vol. 32 (03): 272-276 [Abstract] ( 575 ) [HTML 1KB] [ PDF 3111KB] ( 556 )
江苏大学学报:医学版
　

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