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| Leucine-rich repeat sequence protein 1 promotes breast cancer cell migration and invasion by upregulating the expression of#br#
mammalian rapamycin target proteins |
| WANG Le 1, ZHU Xiaoren 1, PENG Shiqing 1, CHEN Minbin 2 |
| （1. School of Medicine, Jiangsu University, Zhenjiang Jiangsu 212013; 2. Department of Oncology, Kunshan First People′s Hospital Affiliated to Jiangsu University, Kunshan Jiangsu 215300, China） |
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Abstract Objective: To investigate the expression of leucinerich repeat protein 1 （LRR1） in breast cancer cells and its effect on cells biological function. Methods: Human breast cancer MDA-MB-231, HCC70 cell lines and normal breast epithelial MCF-10A cells were cultured; Western blotting was used to detect the expression of LRR1. Human breast cancer MDA-MB-231 and HCC70 cell lines were divided into interference control group, LRR1 interference group, LRR1 overexpression group and empty vector control group, which were transfected with LRR1 control, interference, overexpression and empty vector sequences, respectively; Transwell assay was used to detect migration and invasion ability of breast cancer cells; Western blotting was used to detect the expression of mammalian target of rapamycin （mTOR）. Results: Compared with normal human breast epithelial MCF-10A cells, the expression level of LRR1 in breast cancer MDF-MB-231 and HCC70 cells was significantly increased （both P<0.01）. In human breast cancer MDA-MB-231, HCC70 cell line, compared with the interference control group, LRR1 interference group significantly decreased the number of cell migration and invasion （all P<0.01）, and the expression level of m-TOR was significantly decreased（both P<0.01）; compared with the empty vector control group, LRR1 overexpression group significantly increased the numbers of migration and invasion（both P<0.01）, and the expression level of m-TOR protein was significantly increased （both P<0.01）. Conclusion: LRR1 is highly expressed in breast cancer cell lines and promotes their migration and invasion.
［Key words］breast cancer; leucinerich repeat protein 1; mammalian target of rapamycin（m-TOR）; cell migration; cell invasion
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Received: 03 July 2021
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