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Journal of Jiangsu University(Medicine Edition)

	2021 Vol.31 Issue.04 Published 2021-07-30

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		2021 Vol. 31 (04): 0- [Abstract] ( 44 ) [HTML 1KB] [ PDF 379KB] ( 498 )

	277	microRNA-1290 promotes migration and invasion of colorectal carcinoma cell via NDRG1/NF-κB/IL-6 pathway
	microRNA-1290 promotes migration and invasion of colorectal carcinoma cell via NDRG1/NF-κB/IL-6 pathway[J]. Journal of Jiangsu University(Medicine Edition), 2021,31(04): 277-283>')" href="#">	MO Hui, XU Min
		Objective: To investigate the mechanism of microRNA-1290 （miR-1290） mediating the activation of NF-κB/interleukin-6 （IL-6） signaling by NMyc downstream-regulated gene 1 （NDRG1）, and further explore its impact on the migration and invasion of colorectal carcinoma cells. Methods: Immunohistochemistry were used to detect the expression of IL6 in the tumors and adjacent tissues in 48 cases of colorectal carcinoma. The expression of IL6 and miR-1290 in 20 samples selected at random from the 48 cases, colorectal carcinoma cells and normal colonic epithelial cells, HCoEpiC were detected by qRT-PCR. Western blotting was performed to detect the expression of p-NF-κB, NF-κB, NDRG1, E-cadherin, and vimentin; ELISA was used to test the levels of IL-6 after treatment with miR-1290 inhibitor or mimic, respectively. With the treatment of small interference RNA-IL-6 （siRNA-IL-6） or neutralizing antibody, the effect of miR-1290 in colorectal carcinoma cell migration was assessed by wound healing test, and cell invasion was tested by Matrigel invasion assay. Results: The expression levels of IL-6 were significantly higher in colon cancer tissues than adjacent normal tissues by immunohistochemical staining （P<0.01）, and there was a remarkably positive correlation between miR-1290 and IL-6 mRNA in colorectal carcinoma tissues （r=0.845 4, P<0.01）. Moreover, the expression of miR-1290 in colon cancer cells were significantly higher than in HCoEpiC cell （all P<0.01）. Upregulation of miR1290 by mimic inhibited the expression of NDRG1 and further induced the activation of NF-κB pathway and epithelial mesenchymal transformation（P<0.01）, while the treatment of miR-1290 inhibitor restored the expression of NDRG1 but increased the expression of p-NF-κB（P<0.01）. The scratch results showed miR-1290 upregulation increased the healing rate of colorectal carcinoma cells, which could be blocked by IL-6 silencing（P<0.01）. Coincidently, the number of invasive cells were elevated after miR-1290 mimic treatment, which could be effectively reversed by neutralizing antibody of IL-6（P<0.01）. Conclusion: miR-1290 promotes migration and invasion of colorectal carcinoma cell through the NF-κB/IL-6 axis, which is caused by targeting inhibition of NDRG1.
		2021 Vol. 31 (04): 277-283 [Abstract] ( 35 ) [HTML 1KB] [ PDF 5863KB] ( 556 )

	284	Expression of C14ORF169 in colorectal cancer and its effect on chemotherapy and prognosis
	Expression of C14ORF169 in colorectal cancer and its effect on chemotherapy and prognosis[J]. Journal of Jiangsu University(Medicine Edition), 2021,31(04): 284-289>')" href="#">	LUO Tingyi, SUN Wei, YANG Tingsong, WANG Shilin, HUO Yuhu, WEI Haihong, XU Bin
		Objective: To explore the expression of C14ORF169 in patients with colorectal cancer, and analyze its impact on efficacy of chemotherapy and prognosis. Methods:C14ORF169 differences in expression level of colorectal adenoma, colorectal cancer and corresponding normal tissues were compared using Oncomine database. The correlation of C14ORF169 expression with patients′clinicopathological characteristics and prognosis was analyzed by using the GSE12945 and GSE17536 colorectal cancer datasets downloaded from the gene expression omnibus （GEO） database. The C14ORF169 over expression and knockdown colorectal cancer cell lines were constructed respectively, and the 5-fluorouracil （5-FU） halfmaximal inhibitory concentration （IC50） was detected by using CCK-8 method. Results: The level of C14ORF169 in colorectal adenomas and colorectal cancer tissues were significantly higher than that in normal tissues （P<0.05）. Overall survival and colorectal cancer specific survival of patients with high expression of C14ORF169 were significantly shorter than those with low C14ORF169 expression （P<0.05）. High expression of C14ORF169 was closely related to AJCC advanced stage of colorectal cancer. The sensitivity of colorectal cancer cells to 5FU increased after C14ORF169 knockdown, while C14ORF169 overexpression cell lines showed enhanced chemoresistance. Conclusion: C14ORF169 is highly expressed in colorectal cancer, which is associated with chemotherapy resistance and poor prognosis.
		2021 Vol. 31 (04): 284-289 [Abstract] ( 30 ) [HTML 1KB] [ PDF 84836KB] ( 625 )

	290	Polyphyllin A directly activates AMPK to induce autophagy in colorectal cancer cells
	Polyphyllin A directly activates AMPK to induce autophagy in colorectal cancer cells[J]. Journal of Jiangsu University(Medicine Edition), 2021,31(04): 290-295>')" href="#">	PENG Peng1,2, YANG Shusheng1,3, XIANG Yuchen1,3, WEN Jun1,2, SI Yuan1,3, LIU Ying1,2,3
		Objective: To investigate the effect of Polyphyllin A （PPA） on autophage of colorectal cancer （CRC） cells and its potential mechanism. Methods:Colorectal cancer RKO and HRT18 cells were selected as cell model. RKO and HRT18 cells were treated with different concentrations of PPA, MTT assay was used to detect the cell viability, and to screen the optimal experimental concentration. Colony formation assay was used to detect cell cloning ability. Western blotting was used to detect the expression of microtubule-associated protein Ⅱ light chain-3 （LC3Ⅱ）, expression of AMPactivated protein kinase （AMPK） and mammalian target of rapamycin （mTOR） and its phosphorylation. In addition, immunofluorescence was used to detect autophagosome formation. Molecular docking was used to analyze the interaction of PPA and AMPK. Results: Compared with the 0 μmol/L group, the cell viability in each concentration group of PPA was significantly reduced（P<0.05）. Compared with the 0 μmol/L group, the cloning ability of the two cell lines was significantly reduced （P<0.05）. With the gradual increase of PPA concentration, the expression of LC3Ⅱ in the two cell lines gradually increased, and autophagosomes aggregated. The expression of total AMPK and mTOR protein did not change significantly（P＞0.05）, while the phosphorylation of AMPK was increased and the phosphorylation of mTOR was decreased （P<0.05）. The molecular docking showed that PPA directly combined with AMPK. Conclusion: PPA induced autophagy in CRC cells by directly targeting and activating AMPK.
		2021 Vol. 31 (04): 290-295 [Abstract] ( 42 ) [HTML 1KB] [ PDF 37798KB] ( 587 )

	296	Screening and verification of hub genes for colon cancer based on bioinformatics
		ZENG Cheng1， QI Guoping1， SHEN Ying1，2， LU Wenbin1，2， DENG Jianzhong1，2， LIU Qian1，2， JIN Jianhua1，2
		Objective: To screen the hub genes of colon cancer by bioinformatics methods and verify them through in vitro experiments to explore potential colon cancer molecular markers. Methods： GSE23878， GSE37182 and GSE74602 data sets were downloaded from the GEO database; R language was used to screen differentially expressed genes （DEGs） in colon cancer and adjacent tissues. DEGs was used to perform GO enrichment analysis and KEGG signaling pathway analysis; proteinprotein interaction（PPI） network of DEGs was constructed by using the STRING database， then the hub genes were screened by Cytoscape software. TCGA database was used to validate expression and prognostic value of hub genes. Finally， the functions of hub genes were further verified by cell transfection and MTT assays. Results： A total of 270 overlapping DEGs were obtained from 3 data sets. GO enrichment analysis showed that DEGs were mainly involved in negative regulation of growth， extracellular matrix organization and extracellular structure organization. KEGG signaling pathways analysis showed that DEGs were mainly enriched in Wnt， NFκB and cell cycle signaling pathways. A total of 11 hub genes were screened through the PPI. It was verified by GEPIA database that MYC， TIMP1， UBE2C， SOX9， AURKA， COL1A1， CDC20， TOP2A and CENPF were highly expressed in colon cancer tissues， while CAV1 and CXCL12 were lowly expressed. Survival analysis showed that high expression of AURKA was positively correlated with overall survival （OS） of colon cancer patients （P<0.05）， high expression of TIMP1 was negatively correlated with OS （P<0.05）， and high expression of COL1A1 was negatively correlated with diseasefree survival of colon cancer patients （P<0.05）. Cell transfection and MTT experiments further verified that overexpression of TIMP1 or AURKA could significantly promote the proliferation of colon cancer cells. Conclusion： A total of 11 hub genes of colon cancer were found being involved in the occurrence of colon cancer. The expression of AURKA and TIMP1 may be related to the prognosis of colon cancer.
		2021 Vol. 31 (04): 296-302 [Abstract] ( 49 ) [HTML 1KB] [ PDF 9165KB] ( 539 )

	303


		2021 Vol. 31 (04): 303-306，313 [Abstract] ( 37 ) [HTML 1KB] [ PDF 3826KB] ( 608 )

	307	NLRP3 inflammasome mediates pyroptosis caused by Listeria monocytogenes infection in mice and macrophages
	NLRP3 inflammasome mediates pyroptosis caused by Listeria monocytogenes infection in mice and macrophages[J]. Journal of Jiangsu University(Medicine Edition), 2021,31(04): 307-313>')" href="#">
		Objective: To investigate the potential mechanism of Listeria monocytogenes （Lm）infectionon pyroptosis in mice and macrophages.Methods: In vivo experiments, C57B/6 mice were divided into Lm group and negative control group（NC group）, then the mice were injected with Lm 5×104 CFU/mouse and sterile PBS via tail vein, respectively. The spleen, liver and brain tissues of mice were collected 24 hours later. The pyroptosis and NLRP3 inflammasome related protein expression were detected by Western blotting. In vitro experiments were divided into Lm group, NC group, LPS+ATP group, Lm+MCC950 group and LPS+ATP+MCC950 group. Multiplicity of infection （MOI）= 10 Lm was used to infect THP-1 derived macrophages, ELISA was used to detect IL-1β from cell supernatant, quantitative real-time PCR （qRT-RCR）and Western blotting were used to detect expression of mRNA and protein about pyroptosis and NLRP3 inflammation. Immunofluorescence （IF）was used to detect ASC-speck and co-localization with NLRP3. Results: After Lm infection of mice and macrophages, the expression level of gasdermin D （GSDMD）protein was significantly increased, and gasdermin D N-terminal （GSDMD-NT）was appeared. The levels of NLRP3, caspase-1, IL-1β, and IL-18 mRNA were significantly increased after Lm infected macrophages at 1 h, 3 h, and 6 h （P<0.05）, and the increase was the most obvious at 3 h. The protein expression of NLRP3, pro-caspase-1, pro-IL-1β and cleavage fragment caspase-1 p20, IL-1β-17 in Lminfected mouse spleen, liver, brain tissue were all significantly increased （P<0.05）.In macrophages by Lm infection, the secretion of IL-1β was increased, the secretion of caspase-1 p20 and IL-1β-17 was detected in the cell supernatant, and the expression of NLRP3, pro-caspase-1, pro-IL-1β protein in the cell lysate was increased （P<0.05）, then ASC-speck was formed and co-localized with NLRP3 protein was observed by fluorescence microscope. After pretreatment with specific NLRP3 inflammasome inhibitor MCC950, GSDMD-NT of macrophages was significantly reduced （P<0.05）. Conclusion: Pyroptosis caused by Lm infection is mainly related to the activation of NLRP3 inflammasome in mice and macrophages.
		2021 Vol. 31 (04): 307-313 [Abstract] ( 37 ) [HTML 1KB] [ PDF 12181KB] ( 464 )

	314	Effects of NFATc1 on intestinal microflora of diabetic atherosclerosis ApoE-/- mice
	Effects of NFATc1 on intestinal microflora of diabetic atherosclerosis ApoE-/- mice[J]. Journal of Jiangsu University(Medicine Edition), 2021,31(04): 314-320>')" href="#">	LIU Jia, SUN Zhen, HOU Lina, QIAN Yongjiang, SHAO Chen, YUAN Wei, WANG Zhongqun
		Objective: To explore the effect of nuclear factor of activated Tcell cytoplasmic 1（NFATc1）on the intestinal microflora of diabetic atherosclerosis ApoE-/- mice. Methods: ApoE-/- mice were used to make diabetic atherosclerosis models by the injection of Streptozotocin. AdenoAssociated Viral Vector was injected into the tail vein to interfere with the expression of NFATc1 in vivo. The mice were sacrificed 8 weeks later, and aorta tissues were separated for Western blotting to verify efficacy of the knockdown of NFATc1 in the aorta. The relative area of atherosclerotic plaque was analyzed by HE staining. Mice feces were collected and nuclear acid were extracted for detecting gene sequence by Illumina MiSeq. Class levels of intestinal microflora of mice were determined through bioinformatics analysis. Results: The mouse models of diabetic atherosclerosis were successfully made. After the NFATc1 knockdown AdenoAssociated Viral Vector was injected into the tail vein, its expression was significantly reduced in the aorta（P＜0.05）.HE staining showed that the formation of atherosclerotic plaques in the aorta was significant, but the relative area of atherosclerotic plaques decreased significantly after interfering with the expression of NFATc1, compared to the negative control group（P<0.05）. The 16S rRNA gene sequencing results showed that the relative abundance of Erysipelotrichi in the intestine of NFATc1 knockdown mice was significantly higher than that of negative control mice（78.82% vs. 32.93%，W=2）. Conclusion: NFATc1 could change the relative abundance of intestinal microflora in diabetic atherosclerosis ApoE-/- mice.
		2021 Vol. 31 (04): 314-320 [Abstract] ( 36 ) [HTML 1KB] [ PDF 4351KB] ( 550 )

	321	Exosomal miR-21 secreted by CAF promotes invasion and metastasis of bladder cancer cells
	Exosomal miR-21 secreted by CAF promotes invasion and metastasis of bladder cancer cells[J]. Journal of Jiangsu University(Medicine Edition), 2021,31(04): 321-325，332>')" href="#">	WEI Yong1,2, CHEN Peng1, ZHU Hongru1, CHEN Quanbing1, JIANG Yazhi1, QIAN Wenhui1
		Objective: To explore the role and potential mechanism of exosomal involved in process of cancerassociated fibroblast （CAF） promoting the invasion and metastasis of bladder cancer cells. Methods: CAF and normal fibroblast （NF） was isolated from bladder cancer and normal tissues adjacent to the cancer, respectively. The expression of was assessed in tissues and cell lines of bladder cancer, CAF, NF and exosomes. The transwell invasion and metastasis assays were applied to test the effect of CAF, CAF induced exosomes and on the invasion and metastasis ability of bladder cancer cells. The confirmation of target gene of was performed with luciferase assay. Results: Compared with controls, was highly expressed in bladder cancer tissues, especially those with late stages. Exosomes released from CAF showed higher expression of than that from NF. Incubation with CAF, CAF secreted exosomes, or transfection of mimics could significantly enhance the ability of T24 cells to invade and migrate. Exosomal absorbed by bladder cancer cells could promote the invasion and metastasis by targeting PTEN. Conclusion: Exosomal could regulate the process of CAF promoting the invasion and metastasis of bladder cancer cells.
		2021 Vol. 31 (04): 321-325，332 [Abstract] ( 39 ) [HTML 1KB] [ PDF 4479KB] ( 501 )

	326	Effect of hypoxia on TWIST expression in PANC-1 cells
		MAO Zhengfa1, LUO Junyi2, LI Xi2, MA Xiaoyan3
		Objective: To investigate the effect of hypoxia on the expression of TWIST in PANC-1 cells and its underlying mechanism. Methods: PANC-1 cells were cultured under low oxygen concentration, the expression of TWIST was detected by Western blotting at different time points, and the expression of TWIST, Ecadherin, α-SMA and Vimentin was detected by immunofluorescence; The expression of E-cadherin, α-SMA and Vimentin was detected by Western blotting under hypoxic conditions. After transfection of TWIST siRNA, the expression of E-cadherin, αSMA and Vimentin was detected by Western blotting under normoxic and hypoxic conditions.The expression of METTL3 was detected by qRT-PCR and Western blotting, the total m6A level of cells was detected by colorimetric method under low oxygen concentration; after transfection of METTL3 siRNA, the total m6A level of cells was examined by colorimetric method under normoxia and hypoxia.The expression level of TWIST was detected by qRT-PCR and Western blotting, and the level of TWIST mRNA m6A was detected by MeRIP-qPCR after transfection of METTL3 siRNA under hypoxic conditions; the expression of TWIST protein was detected by Western blotting after transfection of METTL3 wild-type and mutant plasmids. Results: Hypoxia promoted the expression of TWIST, α-SMA and Vimentin while the expression of E-cadherin was decreased in pancreatic cancer cells; at the same time the expression of E-cadherin increased and the expression of α-SMA and Vimentin decreased when knockingdown the expression of TWIST. The mRNA and protein expression of RNA methyltransferase METTL3 were significantly increased under hypoxia treatment, and the total m6A level of the cells was also significantly increased in pancreatic cancer cells. Under normoxia, knockdown of METTL3 expression slightly reduced the m6A level of pancreatic cancer cells, while under hypoxic conditions m6A level was significantly inhibited. Knockdown of METTL3 expression had no significant effect on the expression level of TWIST mRNA in pancreatic cancer cells under hypoxic conditions, but it significantly reduced the m6A level of TWIST mRNA and inhibited the expression of TWIST protein; overexpression of METTL3 significantly increaseed the expression of TWIST protein while not METTL3 mutant plasmid （METTL3-MUT）. Conclusion: Hypoxia could induce the expression of METTL3, which regulates the expression of TWIST protein through an m6A-dependent manner.
		2021 Vol. 31 (04): 326-332 [Abstract] ( 43 ) [HTML 1KB] [ PDF 3154KB] ( 516 )

	333	Mibefradil enhances inhibition of 3, 3′-diindolylmethane in hepatocellular carcinoma cells
	Mibefradil enhances inhibition of 3, 3′-diindolylmethane in hepatocellular carcinoma cells[J]. Journal of Jiangsu University(Medicine Edition), 2021,31(04): 333-338>')" href="#">	JIANG Yuanyue1,2, XU Wei1, GU Jie3, YE Yang1, XU Xinming4, SUN Jun4, CHEN Jian4, LU Rongzhu1,5
		Objective: To investigate the effect of Ttype calcium channel blocker mibefradil on anticancer effects of 3, 3′diindolylmethane（DIM） and its potential molecular mechanism in human hepatocellular carcinoma cells. Methods: SMMC7721 and HepG2 hepatocellular carcinoma cells were cultured in four groups, control group, cultured in high glucose medium containing 10% fetal bovine serum for 24.5 h; mibefradil group, cells were treated with 5 μmol/L mibefradil for 24.5 h; DIM group, cells were treated with 60 μmol/L DIM for 24.5 h; mibefradil+DIM group, cells were pretreated with 5 μmol/L mibefradil for 0.5 h, then cotreated with 5 μmol/L mibefradil and 60 μmol/L DIM in the following 24 h. The morphological changes were observed by the inverted microscope; the cell viability was detected by CCK8 assays; the level of apoptosis was detected by Hoechst 33342 staining; the expression of PCNA, cleavedcaspase 3 and p-p38 MAPK were evaluated by Western blotting; Fluo-3/AM was used as the probe to assess the level of cytosolic free calcium［Ca2+］i. Results: Compared with the control group, the treatment with DIM decreased the cell viability and the expression of PCNA, whereas enhanced the expression of cleaved-caspase 3 and pp38 MAPK（all P<0.05）; the cell morphology was destroyed and the［Ca2+］i fluorescence was enhanced. Compared with the DIM group, the cell viability and the expression of PCNA was lower in mibefradil+DIM group; the level of apoptosis, the expression of cleavedcaspase 3 and pp38 MAPK was further increased（all P<0.05）; cell morphology was further destroyed, the level of ［Ca2+］i was muh higher. Conclusion: Ttype calcium channel blocker mibefradil could enhance the anticancer effects of DIM through the increase of［Ca2+］i and pp38 MAPK.
		2021 Vol. 31 (04): 333-338 [Abstract] ( 52 ) [HTML 1KB] [ PDF 3353KB] ( 478 )

	339	Contents of IL-15, IL-18, IL-21 and NK cells in peripheral blood of patients with syphilis serofast and its clinical significance
	Contents of IL-15, IL-18, IL-21 and NK cells in peripheral blood of patients with syphilis serofast and its clinical significance[J]. Journal of Jiangsu University(Medicine Edition), 2021,31(04): 339-344>')" href="#">	CHENG Wenhao, LU Yumo, HU Wenlong, LIU Zhonglun, REN Hong
		Objective: To investigate the changes of IL15, IL18, IL21 and NK cells in peripheral blood of patients with syphilis serofast and its clinical significance. Methods: A total of 24 patients with syphilis serofast treated in Affiliated Lianyungang Hospital of Xuzhou Medical University from October 2018 to September 2020 were selected as the syphilis serofast group, and 24 healthy people who underwent physical examination were taken as the healthy control group. The levels of NK cells of two groups were measured by flow cytometry, and levels of IL-15, IL-18 and IL-21 of two groups were detected by double antibody and sandwich ELISA. The available network databases were searched, and papers on the correlation between NK cells and syphilis serofast which met the established standards were chosen to conduct Meta analysis. Results: The levels of IL-18, IL-21 and the percentage of NK cells in the peripheral blood of the serofast group were significantly lower than those of the healthy control group （P＜0.01), and there was no significant difference in the levels of IL-15 between the two groups （P=0.173）. Meta analysis results showed that compared with healthy controls, the percentage of NK cells in peripheral blood of syphilis serofast patients was significantly reduced［SMD=-0.64, 95% CI（-1.08, -0.21）, P＜0.001］. Conclusion: In patients with syphilis serofast, the percentage of NK cells in peripheral blood decreased and the immune function of the body was inhibited,which may be one of the reasons for the formation of serofast.
		2021 Vol. 31 (04): 339-344 [Abstract] ( 43 ) [HTML 1KB] [ PDF 2094KB] ( 577 )

	345	Application value of N-terminal B-type brain natriuretic peptide and procalcitonin in prognostic evaluation of patients with acute cardiac infarction
	Application value of N-terminal B-type brain natriuretic peptide and procalcitonin in prognostic evaluation of patients with acute cardiac infarction[J]. Journal of Jiangsu University(Medicine Edition), 2021,31(04): 345-349>')" href="#">	XU Fei， JIANG Ya， LIANG Jianjun
		Objective: To explore the application value of Nterminal Btype brain natriuretic peptide （NTproBNP） and procalcitonin （PCT） in the prognostic evaluation of patients with acute myocardial infarction. Methods： A total of 260 patients with acute cardiac infarction and 248 healthy persons were included in this study. Logistic regression analysis and receiver operating characteristic curve （ROC） were used to determine the application value of serum NTproBNP and PCT levels. Results： Serum NTproBNP and PCT levels in acute myocardial infarction patients were higher than those in healthy controls （P<0.01）. Serum NT-proBNP and PCT levels of patients who died of acute cardiac infarction were higher than those of surviving control （P<0.01）. Comparing the independent composition ratio of age and Killip cardiac function classification， the difference was statistically significant （P<0.01）; Logistic multivariate analysis showed that age > 60 years old， Killip cardiac function grade Ⅲ to Ⅳ， serum NT-proBNP level， and serum PCT level were all independent risk factors affecting the death in patients with acute myocardial infarction （P<0.01）. ROC analysis showed that the best cut-off points for serum NT-proBNP and PCT levels were 228.95 ng/L and 1.12 ng/mL， respectively， the sensitivity， specificity and area under the curve （AUC） of the combination of the two were 79.17%， 99.58% and 0.861， respectively， and the specificity and AUC wee the highest （specificity， NT-proBNP： P<0.01， PCT： P<0.01; AUC， NT-proBNP： P<0.01， PCT： P<0.01）. Conclusion： Serum NT-proBNP and PCT levels in patients with acute cardiac infarction are abnormally elevated， and both are independent risk factors for patients death， the combination of the two are more effective in predicting the prognosis of the disease.
		2021 Vol. 31 (04): 345-349 [Abstract] ( 41 ) [HTML 1KB] [ PDF 756KB] ( 528 )

	350	Mechanism of Xuanbai Qingfei Jiedu Decoction in the treatment of COVID19 based on network pharmacology
	Mechanism of Xuanbai Qingfei Jiedu Decoction in the treatment of COVID19 based on network pharmacology[J]. Journal of Jiangsu University(Medicine Edition), 2021,31(04): 350-355>')" href="#">	JU Hongye1，2， ZHAO Guowang1， HAN Lizhu2， WANG Xiaoling1， CUI Xiang1，
		Objective： To explore the pharmacological mechanism of Xuanbai Qingfei Jiedu Decoction in the treatment of coronavirus disease 2019 （COVID19） on account of network pharmacology. Methods： The TCMSP database was used to predict all the active constituents and action targets of 13 kinds of Chinese medicine from Xuanbai Qingfei Jiedu Decoction. The gene names of the key targets were obtained by the UniProt database， and the Cytoscape 3.7.2 network was used to construct the compounddiseasetarget network. The GeneCards database was applied to search the genes associated with novel coronavirus. Meanwhile， the relevant targets were screened by the Venny platform. The PPI network map of core targets was constructed by STRING database and the enrichment analysis of GO and KEGG was implemented by David database. In addition， the enrichment results were visualized by R software. Results： After analysis of data，122 compounds were screened from Xuanbai Qingfei Jiedu Decoction， involving 638 targets， 26 nodes， 3 core networks ，and 35 destined targets were analyzed by GO enrichment. A total of 138 biological processes （P<0.01）were acquired， including exogenous apoptosis signal pathway， drug reaction， lipopolysaccharide mediated signal pathway， etc. Thirtyfive core targets pathways were enriched by KEGG （P<0.01）， the eight groups related to diseases were exhibited： tuberculosis， hepatitis B， trypanosomiasis， etc. The related pathway information were displayed，such as cancer pathway， PI3K-Akt signaling pathway， tumor necrosis factor signaling pathway and other important pathway. The molecular docking results showed that the binding energies of the active components and COVID-19related targets（ACE2） were all less than -5 kJ/mol and fairly stable. Conclusion： This study revealed the potential active chemical components and possible mechanism of action of Xuanbai Qingfei Jiedu Decoction in the treatment of COVID19.
		2021 Vol. 31 (04): 350-355 [Abstract] ( 34 ) [HTML 1KB] [ PDF 7706KB] ( 527 )

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		2021 Vol. 31 (04): 356-362 [Abstract] ( 36 ) [HTML 1KB] [ PDF 2376KB] ( 546 )

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		2021 Vol. 31 (04): 363-368 [Abstract] ( 34 ) [HTML 1KB] [ PDF 753KB] ( 569 )

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