重楼皂苷A直接靶向活化AMPK诱导结直肠癌细胞自噬

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Abstract Objective: To investigate the effect of Polyphyllin A （PPA） on autophage of colorectal cancer （CRC） cells and its potential mechanism. Methods:Colorectal cancer RKO and HRT18 cells were selected as cell model. RKO and HRT18 cells were treated with different concentrations of PPA, MTT assay was used to detect the cell viability, and to screen the optimal experimental concentration. Colony formation assay was used to detect cell cloning ability. Western blotting was used to detect the expression of microtubule-associated protein Ⅱ light chain-3 （LC3Ⅱ）, expression of AMPactivated protein kinase （AMPK） and mammalian target of rapamycin （mTOR） and its phosphorylation. In addition, immunofluorescence was used to detect autophagosome formation. Molecular docking was used to analyze the interaction of PPA and AMPK. Results: Compared with the 0 μmol/L group, the cell viability in each concentration group of PPA was significantly reduced（P<0.05）. Compared with the 0 μmol/L group, the cloning ability of the two cell lines was significantly reduced （P<0.05）. With the gradual increase of PPA concentration, the expression of LC3Ⅱ in the two cell lines gradually increased, and autophagosomes aggregated. The expression of total AMPK and mTOR protein did not change significantly（P＞0.05）, while the phosphorylation of AMPK was increased and the phosphorylation of mTOR was decreased （P<0.05）. The molecular docking showed that PPA directly combined with AMPK. Conclusion: PPA induced autophagy in CRC cells by directly targeting and activating AMPK.

Received: 17 December 2020

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	PENG Peng1
	2
	YANG Shusheng1
	3
	XIANG Yuchen1
	3
	WEN Jun1
	2
	SI Yuan1
	3
	LIU Ying1
	2
	3

Cite this article:

PENG Peng1,2,YANG Shusheng1等.

Polyphyllin A directly activates AMPK to induce autophagy in colorectal cancer cells

[J]. Journal of Jiangsu University(Medicine Edition), 2021, 31(04): 290-295.

URL:

http://zzs.ujs.edu.cn/xbyxb/EN/ OR http://zzs.ujs.edu.cn/xbyxb/EN/Y2021/V31/I04/290

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