[an error occurred while processing this directive]
Journal of Jiangsu University(Medicine Edition)
 Home | Instruction for Authors | About Journal | Subscriptions | Advertisement | Contacts Us | 中文
 
　

Office Online

 　
　  Author Center
　  Peer Review
　  Editor Work
　  Office Work
　  Editor-in-chief
　

Journal Online

 　
　  Forthcoming Articles
　  Current Issue
　  Next Issue
　  Archive
　  Read Articles
　  Download Articles
　  Email Alert
　  
Quick Search  
  Advanced Search
Journal of Jiangsu University(Medicine Edition)
 
2020 Vol.30 Issue.05
Published 2020-09-30
1
2020 Vol. 30 (05): 1- [Abstract] ( 31 ) [HTML 1KB] [ PDF 705KB] ( 551 )
369
2020 Vol. 30 (05): 369-375 [Abstract] ( 26 ) [HTML 1KB] [ PDF 1171KB] ( 813 )
376
2020 Vol. 30 (05): 376-385 [Abstract] ( 25 ) [HTML 1KB] [ PDF 2866KB] ( 809 )
386
2020 Vol. 30 (05): 386-389 [Abstract] ( 31 ) [HTML 1KB] [ PDF 716KB] ( 767 )
390
2020 Vol. 30 (05): 390-394 [Abstract] ( 36 ) [HTML 1KB] [ PDF 730KB] ( 680 )
395
Analysis of viral community in bronchoalveolar lavage fluid of patients with fever of unknown origin
Analysis of viral community in bronchoalveolar lavage fluid of patients with fever of unknown origin[J]. Journal of Jiangsu University(Medicine Edition), 2020,30(05): 395-401>')" href="#"> LI Xin-lin1, LIU Qi1, CHEN Xu1, XIAO Yu-qing1, YANG Shi-xing1, ZHANG Wen1, CHEN Jian-guo2
Objective: To analyze the virus profile in alveolar lavage fluid of patients with fever of unknown origin. Methods: A total of 58 samples of alveolar lavage fluid were randomly divided into five groups to construct 5 libraries.  Virus metagenomics technology was used to analyze the virus community of five libraries, and PCR screening was performed for the obtained torque teno virus and rhinovirus. Results: The results of virus composition showed that the virus community was dominated by Anelloviridae, which accounted for 19.4% of the total population, followed by Circoviridae(11.0%), Phycodnaviridae(11.0%), Human endogenous retrovirus(10.2%), Poxviridae(4.9%), Retroviridae(3.2%),Virgaviridae(2.3%) and Picornaviridae(0.6%). Four strains of torque teno virus with long sequences were found, and phylogenetic analysis was carried out according to open reading frame 1 of torque teno virus, which showed that it was different from the known virus in some sequences. The positive rate of torque teno virus and rhinovirus was 11.3% and 10.0% respectively by PCR screening. Conclusion: The viral community composition in alveolar lavage fluid of patients with fever of unknown origin was determined with virus metagenomics technology. The Anelloviridae had the highest proportion in the virus community, in which the torque teno virus proliferated due to the inflammatory environment caused by fever and the low immunity of patients.
2020 Vol. 30 (05): 395-401 [Abstract] ( 53 ) [HTML 1KB] [ PDF 3576KB] ( 677 )
402
Protection of ischemic post-conditioning against spinal cord ischemia-reperfusion injury and its effect on the expression of
calciumsensing receptor and Caspase-12 in rats
Protection of ischemic post-conditioning against spinal cord ischemia-reperfusion injury and its effect on the expression of
calciumsensing receptor and Caspase-12 in rats
[J]. Journal of Jiangsu University(Medicine Edition), 2020,30(05): 402-407,413>')" href="#">		 ZHANG Rui1， SUN Ji-fu2， CHEN Qian1， CAO Su-cheng1， HUANG Yong-hui2
Objective: To investigate the protective effect of ischemic post-conditioning（IPC） on spinal cord ischemia reperfusion injury（SCIRI） in rats and to observe its effect on the expression of calciumsensing receptor（CaSR） and endoplasmic reticulum stress-related proteins Caspase-12. Methods: Fifty SD rats were randomly and averagely divided into 5 groups: sham operation group (Sham group)，ischemia-reperfusion model group（IR group）,IR model+IPC group (IPC group), IR model +IPC+agonist (IPC-GdCl3 group) and IR model+inhibitor group(NPS2390 group). Sham group only exposed abdominal aorta but not clamping, SCIRI models were established after clamping abdominal aortic for 25 min and then openning it in other groups, IPC group was treated with 3 circulatory IPC after 3 min perfusion, IPC-GdCl3 group was treated with IPC group’s treatment after tail vein injection of CaSR agonist GdCl3 for 2 days. CaSR inhibitor NPS2390 was injected intravenously in NPS2390 group once a day for 2 days before establishing IR model. The motor function of hindlimb was evaluated by BBB score, the apoptosis of spinal cord tissue was detected by TUNEL staining, and the expression of CaSR and Caspase-12 was detected by Western blotting and immunohistochemistry. Results: The BBB scores of the model groups were significantly lower than those of the sham group (P<0.05). The scores of the IPC group and the NPS2390 group were higher than those of the IR group(P<0.05). The scores of the IPC group were higher than those of the IPC-GdCl3 group (P<0.05). TUNEL staining showed that almost no apoptotic nerve cells were found in sham group, and the number of apoptosis in IR group were higher than that in IPC group and NPS2390 group (P<0.05). The number of apoptosis in IPC group were lower than that in  IPC-GdCl3 group (P<0.05).Western blotting results showed that the expression of CaSR and Caspase12 protein in the model group was significantly higher than that in the sham group (P<0.05).The expression of CaSR and Caspase12 in IR group was higher than that in IPC group and NPS2390 group (P<0.05).The expression of CaSR and Caspase-12 in IPC group was lower than that in IPCGdCl3 group (P<0.05). The results of immunohistochemistry were consistent with Western blotting. Conclusion: IPC exerts a protective effect on SCIRI in rats and this protection may be attributed to inhibition of CaSR and Caspase12 expression in spinal cord tissues.
2020 Vol. 30 (05): 402-407,413 [Abstract] ( 38 ) [HTML 1KB] [ PDF 2585KB] ( 631 )
408
RAC1 inhibits the migration and invasion of human epithelial ovarian cancer cells by regulating the distribution of tight junction protein 1
RAC1 inhibits the migration and invasion of human epithelial ovarian cancer cells by regulating the distribution of tight junction protein 1[J]. Journal of Jiangsu University(Medicine Edition), 2020,30(05): 408-413>')" href="#">
Objective: To investigate the effects and mechanism of Rac family small GTPase 1 (RAC1) on the migration and invasion of ovarian cancer cells. Methods: The pLKO.1puro RAC1 shRNA lentiviral vector was constructed to package lentivirus and infect ovarian cancer cell lines OVCAR3 and SKOV3 which were selected by puromycin. Western blotting was performed to determine the RAC1 knockdown efficiency. Wound healing assay and matrigel invasion assay were applied to detect the migration and invasion. The protein expression and distribution of tight junction protein 1 (TJP1) were visualized by Western blotting and immunefluorescence staining. Results: Restriction enzyme digestion and sequencing confirmed the successful construction of RAC1 shRNA lentiviral vector. Western blotting showed the stable RAC1 knockdown in OVCAR3 and SKOV3 (P<0.05). After knocking down RAC1, the cell migration and invasion were significantly decreased (all P<0.01). Western blotting confirmed that RAC1 knockdown in OVCAR3 and SKOV3 did not affect TJP1 protein expression, but relocated TJP1 distribution from the cytoplasm to cellcell junction and strengthened the connection between cells. Conclusion: RAC1 knockdown might inhibit the migration and invasion of ovarian cancer cells by regulating the intercellular distribution of TJP1.
2020 Vol. 30 (05): 408-413 [Abstract] ( 39 ) [HTML 1KB] [ PDF 2218KB] ( 792 )
414
Changes of granulocytic myeloid-derived suppressor cell and Th17 cells in spleen cells of asthmatic mice
Changes of granulocytic myeloid-derived suppressor cell and Th17 cells in spleen cells of asthmatic mice[J]. Journal of Jiangsu University(Medicine Edition), 2020,30(05): 414-417>')" href="#"> XUE Fei1,2, YU Meng-zhu1,2, SHAN Wenqi1,2, LU Hong-yan1, WANG Xue-feng2
Objective: To explore the level of myeloid-derived suppressor cells(MDSCs), Th17 and Treg cells in spleen cells of asthmatic mice. Methods: BALB/c male mice at age of 6-8 weeks were randomly divided into two groups: PBS group and model group. The group of asthmatic mice was established with OVA, and the ova-specific IgE in the mouse serum was detected by enzyme-linked immunosorbent assay (ELISA). HE staining and PAS staining were used to observe the pathological change in lung tissues. The levels of Th17, Treg and MDSCs in splenocyte were detected by flow cytometry. Immunohistochemical staining was used to observe the expression of Gr-1, IL-17 and Foxp3 in lung tissues of mice. Results: Compared with PBS group, airway inflammatory cells and mucus secretion were increased in the model group and serum IgE level was significantly increased. The proportion of Th17 cells in spleen cells increased and that of Treg cells decreased. The percentage of G-MDSCs subset in MDSCs was increased. Meanwhile, IL-17 and Gr-1 were highly expressed and Foxp3 was low in lung tissue of model group. Conclusion: High proportion of G-MDSCs and Th17 cells, and low proportion of Treg may be contributed to pathogenesis of asthmatic mice.
2020 Vol. 30 (05): 414-417 [Abstract] ( 44 ) [HTML 1KB] [ PDF 11655KB] ( 583 )
418
Effects of Baicalin on the stimulator of interferon gene pathway activation in murine bone marrow-derived macrophages
Effects of Baicalin on the stimulator of interferon gene pathway activation in murine bone marrow-derived macrophages[J]. Journal of Jiangsu University(Medicine Edition), 2020,30(05): 418-422>')" href="#"> GAO Feng-wei， JIANG Meng-ying， ZHANG Xin-yi， Lv Yun-wei， JIANG Ge-chen， YU Wan-jun，
Objective: To determine the effects of Baicalin on stimulator of interferon genes （STING）pathway in murine bone marrowderived macrophages（BMDMs）.  Methods: The murine bone marrow cells were used to induce BMDMs by macrophage colony stimulating factor（M-CSF）in vitro. Then, differentiated BMDMs were divided into six groups in the experiment: dimethyl sulfoxide（DMSO）control group, Baicalin group, cGAMP group, Baicalin+cGAMP group, LPS group and Baicalin+LPS group. The morphological characteristics of the BMDMs were observed under the microscope. The expression of CD80 and CD86 on BMDMs were detected by flow cytometry. The production of nitric oxide（NO）in the supernatant of BMDMs was determined through Griess method. The levels of IL6, TNFα and IFN-β in supernatant of BMDMs were analyzed using ELISA. Results: Baicalin treatment had no significant effects on the morphology and growth status of BMDMs, but it significantly inhibited nitric oxide secretion and the expression of CD80 and CD86 in BMDMs. Moreover, it also inhibited the secretion of IL6, TNFα and IFNβ in activated macrophages, through depressing the STING pathway in BMDMs. Conclusion: Baicalin restrains the activation of STING pathway which induced by cGAMP in BMDMs.
2020 Vol. 30 (05): 418-422 [Abstract] ( 39 ) [HTML 1KB] [ PDF 1938KB] ( 636 )
423
Autophagy and apoptosis induced by OmpA of Acinetobacter baumannii via ROS/NLRP3 signaling pathway in THP-1 cells
Autophagy and apoptosis induced by OmpA of Acinetobacter baumannii via ROS/NLRP3 signaling pathway in THP-1 cells[J]. Journal of Jiangsu University(Medicine Edition), 2020,30(05): 423-429>')" href="#"> DAI Xiao-yue1, WU Liang1, XIA Wen1, YIN Qing2, ZOU Zhi-qing1, ZHOU Ya-ling2, HE Lei2, DING Long-kun1, XI Yue1,
Objective: To investigate the effect of outer membrance protein A(OmpA) of Acinetobacter baumannii on autophagy and apoptosis of human monocyte macrophage THP-1 cells and its underlying mechanism. Methods: The OmpA/pET-28a vector was constructed and transformed into E.coli BL21(DE3) to express the recombinant OmpA (rOmpA), and the rabbits were immunized by rOmpA to prepare the polyclonal anti-OmpA antibody. The anti-OmpA antibody levels in serum of ventilatorassociated pneumonia(VAP) patients were detected by Western blotting. THP-1 cells were treated with different concentrations(1 μg/mL and 10 μg/mL) of rOmpA for 1 h, 3 h and 6 h; and the expression of autophagy-related proteins LC-3 and Beclin-1 were detected by Western blotting; the flow cytometry was used to detect the THP-1 cell apoptosis rate and ROS production; the mRNA expressions of NLRP3, Caspase-1 and IL-1β in THP-1 cells were detected by qRT-PCR. Results: The OmpA expression in clinical A. baumannii strains could be detected by polyclonal anti-OmpA antibody, and the anti-OmpA antibody (IgG) in serum of VAP patients serum could be detected before and after the onset of VAP. The levels of anti-OmpA antibody in VAP patients′ serum were significantly higher than that before using tracheal intubation (P<0.05). After treated with rOmpA, the expression of autophagy-related proteins LC3-Ⅱ and Beclin-1 in THP-1 cells increased significantly, and the apoptosis rate increased vastly in a time- and concentration-dependent manner (P<0.05). Compared with the control group, when cells were treated by the rOmpA for 1 h, the IL-1β mRNA expression and reactive oxygen species were significantly up-regulated (P<0.05); when treated for 3 h and 6 h, the three kinds of mRNA expression had a significant decrease compared with the control group (P<0.05). Conclusion: OmpA induces THP-1 cells autophagy and apoptosis by activating NLRP3 inflammatory cytokines and releasing inflammatory factors such as ROS and IL-1β.
2020 Vol. 30 (05): 423-429 [Abstract] ( 28 ) [HTML 1KB] [ PDF 2426KB] ( 643 )
430
Protein arginine methyltransferase 5 promotes the migration of human ovarian cancer HO8910 cells
Protein arginine methyltransferase 5 promotes the migration of human ovarian cancer HO8910 cells[J]. Journal of Jiangsu University(Medicine Edition), 2020,30(05): 430-434>')" href="#"> ZHAO Kang-rong, SUN Ai-qin, LIN Qiong, SHAO Gen-bao
Objective: To investigate the effect of protein arginine methyltransferase 5(PRMT5) on epithelial-to-mesenchymal transition(EMT) and migration ability in human ovarian cancer HO8910 cells. Methods: PRMT5 overexpressed HO8910 cell line was established by transient transfection method, and divided into blank control group (wild-type HO8910 cell line), negative control group (HO8910 cells transfected with pCMV-Myc plasmid), and experimental group (HO8910 cells transfected with pCMV-Myc-PRMT5 plasmid). At the same time, HO8910 cell lines with stable PRMT5 knockdown were established by shRNA interference and divided into pLKO control group (infected with empty lentivirus), pKLO+Dox group (100 ng/mL), shPRMT5 group (infected with PRMT5 shRNA lentivirus) and shPRMT5+Dox group (100 ng/mL). The mRNA expression and interference efficiency of PRMT5 were detected by Western blotting or qRT-PCR. The experiment was divided into shPRMT5 group, shPRMT5+Dox group, pCMV-Myc group and pCMV-Myc-PRMT5 group; Transwell assay was used to detect the cell migration ability. The expression of EMT-related proteins was detected by Western blotting. Results: Western blotting and qRT-PCR verify the establishment of ovarian cancer HO8910 cell lines with PRMT5 overexpression and stable knockdown PRMT5.After PRMT5 knockdown, the mobility of ovarian cancer HO8910 cells was significantly decreased (all P<0.01), and the expression of mesenchymal marker related molecules including matrix metalloproteinase-2, vimentin, and N-cadherin was down-regulated, and the expression of epithelial marker E-cadherin was up-regulated.In addition, exogenous PRMT5 expression in ovarian cancer cells greatly up-regulated the migration ability of HO8910 cells (all P<0.05). Conclusion: PRMT5 could promote the EMT process of ovarian cancer HO8910 cells and enhance cell migration.
2020 Vol. 30 (05): 430-434 [Abstract] ( 42 ) [HTML 1KB] [ PDF 1860KB] ( 678 )
435
Effect of non-coding RNA GlmY on biofilm formation of Salmonella enterica serovar Typhi
Effect of non-coding RNA GlmY on biofilm formation of Salmonella enterica serovar Typhi[J]. Journal of Jiangsu University(Medicine Edition), 2020,30(05): 435-438>')" href="#"> LI Xue1, ZHAO Xin2, JIN Meng-tong1, TANG Hao1, ZHANG Ying1, HUANG Xin-xiang1
Objective: To investigate the effect of non-coding RNA GlmY on the biofilm formation of Salmonella enterica serovar Typhi (S. Typhi) and its potential molecular mechanisms.Methods: The homologous recombination induced by the suicide plasmid pGMB151 was used to generate the glmY deleted mutant of S. Typhi. The bacterial biofilm formation in 96-microwell plate was detected by crystal violet staining. qRT-PCR was used to analyze the mRNA expression levels of biofilm formation-related genes in glmY deleted mutant and wild-type strain. Results: The glmY deletion mutant strain was successfully constructed. Compared with the wild-type strain, the glmY deletion mutant showed significantly weaker ability of biofilm formation. The qRT-PCR results showed that mRNA level of csgD and csgA was significantly decreased after deletion of GlmY. Conclusion: The non-coding RNA GlmY may enhance the biofilm formation of S. Typhi by up-regulating the expression of csgD, csgA and Curli synthesis.
2020 Vol. 30 (05): 435-438 [Abstract] ( 36 ) [HTML 1KB] [ PDF 1996KB] ( 645 )
439
Development and validation of a radiomic nomogram for preoperative Lauren classification in gastric cancer
Development and validation of a radiomic nomogram for preoperative Lauren classification in gastric cancer[J]. Journal of Jiangsu University(Medicine Edition), 2020,30(05): 439-444>')" href="#"> DING Yi1, LU Chao2, WANG Xiao-xiao2, CHEN Jian1, SHAN Xiu-hong2
Objective: To explore the feasibility of CT-based radiomics nomogram to preoperatively differentiate Lauren diffuse type from intestinal type in gastric cancer. Methods: The clinical data of 539 patients with pathologically diagnosed gastric cancer were retrospectively analyzed. All patients randomly separated into two cohorts at a 7 ∶3 ratio for training and validation. Venous phase CT images were segmented by radiologists with ITK-SNAP software manually.Two sets of radiomic features were derived from tumor region and peritumor on venous phase CT images.With the least absolute shrinkage and selection operator(LASSO) logistic regression，the effective characteristics were selected. A tumor-based model, a peripheral ring-based model, a combined radiomic signature,clinical model 1 and clinical model 2 were proposed. Afterwards, a radiomic nomogram integrating the combined radiomic signature and clinical characteristics was developed. Receiver operating characteristic (ROC) curves as well as corresponding area under ROC curves (AUC) were estimated for both cohorts to assess the corresponding discrimination ability.We adopted Delong-test to compare the predictive performance between each two models. Calibration curves were conducted to verify the good fitness of model predictive outputs with actual values for the radiomic nomogram. Decision curves were conducted in the validation to quantify the usefulness in clinical trials. Results: The combined radiomic signature achieving an AUC of 0.715 (95% CI： 0.663-0.767) in the training cohort and 0.714 (95%CI： 0.636-0.792) in the validation cohort. The radiomic nomogram incorporating the combined radiomic signature,and clinical characteristics supassed all the other models with a training AUC of 0.745 (95%CI： 0.696-0.795) and a validation AUC of 0.758 (95%CI：0.685-0.831). Further, calibration curves and decision curves demonstrated its great model fitness and clinical usefulness. Conclusion: The radiomic nomogram based on the combined radiomic signature and clinical characteristics held potential in differentiating Lauren diffuse type from intestinal type, which is benefical for reasonable clinical treatment strategy.
2020 Vol. 30 (05): 439-444 [Abstract] ( 40 ) [HTML 1KB] [ PDF 2387KB] ( 761 )
445
Subtype identification of cognitive impairment in first-episode schizophrenia based on factor analysis and cluster analysis
Subtype identification of cognitive impairment in first-episode schizophrenia based on factor analysis and cluster analysis[J]. Journal of Jiangsu University(Medicine Edition), 2020,30(05): 445-450>')" href="#"> HU An-ran, ZENG Jin-kun, HE Tao, YAO Xia, DENG Wei
Objective: Data-driven subtype grouping was conducted for cognitive impairment in the first-episode schizophrenia and subsequently explored the damage differences of independent cognitive dimensions in patients with different subtypes.  Methods: The neurocognitive functions in 253 first-episode schizophrenia patients and 293 healthy controls were evaluated by the Cambridge Neuropsychological Test Automatic Battery (CANTAB). Then, different cognitive dimensions were extracted by factor analysis, and cluster analysis was carried out to group patients with first-episode schizophrenia. At the same time, psychopathological symptoms and the social professional function of the patient group were evaluated by the Positive Negative Syndrome(PANSS) and the Global Assessment Function(GAF) to explore the cognitive impairment mechanism and clinical characteristics of different cognitive subtypes. Results: The neurocognitive functions of the first-episode schizophrenia and the normal control could be divided into seven separable dimensions. Two hundred and fifty-three first-episode schizophrenics were divided into two subgroups: low-function group and high-function group. There were significant differences in cognitive function and clinical symptoms between different subgroups(P<0.01). Compared with the normal controls, the low-function group showed a more severe and extensive cognitive decline, while the highfunction group retained relatively inhibitory function, and the impairment of other cognitive dimensions was between the low-function group and the healthy controls. Conclusion: There are different cognitive subtypes of schizophrenia, and the impairment of inhibitory function is different in different subtypes of schizophrenia, which may be the core marker to identify different cognitive subtypes of first-episode schizophrenia.
2020 Vol. 30 (05): 445-450 [Abstract] ( 49 ) [HTML 1KB] [ PDF 2229KB] ( 712 )
451
Effect of Bacillus licheniformis Capsule on the cytokines level and clinical prognosis of COVID-19 patients
Effect of Bacillus licheniformis Capsule on the cytokines level and clinical prognosis of COVID-19 patients[J]. Journal of Jiangsu University(Medicine Edition), 2020,30(05): 451-454>')" href="#"> YIN Jiang-tao1, TANG Yang2, MEI Qiong1, SUN Zhi-wei1, ZHANG Jin-hui1, LIU Da-dong1,DAI Xiao-yue3, XIA Wen3, DING Long-kun3, XI Yue3, ZHANG Ying3, WU Liang3
Objective: To investigate the drug of Bacillus licheniformis Capsule (BLC) to improve the high levels of cytokines and clinical prognosis in COVID-19 patients, and to provide the clinical evidence for gut microflora therapy in COVID-19. Methods: A retrospective analysis of 38 cases of adult severe COVID-19 patients admitted from February to March 2020，among whom 18 patients received BLC treatment and 20 did not. All patients received regular treatment, the treatment group taking additional BLC treatment every day to improve gut microflora. The basic clinical characteristics and prognosis were recorded in this experiment, as well as counts of white blood cells, lymphocytes, platelets and level of TNF-α, IL-1β, IL-6, PCT, CRP, ALT, albumin and creatinine before and after treatment. Results: After two weeks of treatment, the BLC treatment group and the control group in peripheral blood lymphocyte count was significantly higher than before treatment (P<0.01), serum-CRP levels were significantly lower than before treatment (P<0.01). There was no significant differences in peripheral blood lymphocyte count and serum-CRP levels between the two groups (P>0.05). After BLC treatment for two weeks, TNF-α and IL-1β levels of serum in the BLC treatment group were significantly lower than before treatment, and lower than the control group (P<0.05). But, there were no significant differences in clinical prognosis in two groups (P>0.05). Conclusion: BLC could reduce the levels of TNF-α and IL-1β in serum of COVID-19 patients, but we do not observe a significantly improve of clinical prognosis in patients.
2020 Vol. 30 (05): 451-454 [Abstract] ( 33 ) [HTML 1KB] [ PDF 749KB] ( 656 )
455
2020 Vol. 30 (05): 455-460 [Abstract] ( 25 ) [HTML 1KB] [ PDF 739KB] ( 640 )
江苏大学学报:医学版
　

News

 　
　　　　　　　　        　　More 
　

Links

 　
　　　　　　　　        　　More 
 

Copyright © 2011 Journal of Jiangsu University(Medicine Edition)
Editorial Department of Journal of Jiangsu University   E-mail：xbyx@ujs.edu.cn