RAC1通过调节紧密连接蛋白1分布影响卵巢癌细胞的迁移和侵袭

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Abstract Objective: To investigate the effects and mechanism of Rac family small GTPase 1 (RAC1) on the migration and invasion of ovarian cancer cells. Methods: The pLKO.1puro RAC1 shRNA lentiviral vector was constructed to package lentivirus and infect ovarian cancer cell lines OVCAR3 and SKOV3 which were selected by puromycin. Western blotting was performed to determine the RAC1 knockdown efficiency. Wound healing assay and matrigel invasion assay were applied to detect the migration and invasion. The protein expression and distribution of tight junction protein 1 (TJP1) were visualized by Western blotting and immunefluorescence staining. Results: Restriction enzyme digestion and sequencing confirmed the successful construction of RAC1 shRNA lentiviral vector. Western blotting showed the stable RAC1 knockdown in OVCAR3 and SKOV3 (P<0.05). After knocking down RAC1, the cell migration and invasion were significantly decreased (all P<0.01). Western blotting confirmed that RAC1 knockdown in OVCAR3 and SKOV3 did not affect TJP1 protein expression, but relocated TJP1 distribution from the cytoplasm to cellcell junction and strengthened the connection between cells. Conclusion: RAC1 knockdown might inhibit the migration and invasion of ovarian cancer cells by regulating the intercellular distribution of TJP1.

Received: 16 April 2020

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http://zzs.ujs.edu.cn/xbyxb/EN/ OR http://zzs.ujs.edu.cn/xbyxb/EN/Y2020/V30/I05/408

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