Objective: To prepare the brain-derived neurotrophic factor (BDNF) poly (lactic-coglycolic acid) copolymer (PLGA) nanoparticles (BDNFPLGANPs) and to investigate its properties and biological activities. Methods: PLGA-NPs were prepared by double emulsion-solvent evaporation method.The formulation process was optimized by orthogonal test according to entrapment efficiency(EE) and drug loading. BDNF-PLGANPs were prepared and characterized under optimized conditions. The cell biocompatibility of BDNF-PLGA-NPs was evaluated by Alarma Blue proliferation assay. Results: The optimal conditions for preparation of BDNF-PLGA-NPs included a volume ratio of primary emulsion to external water phase of 1 ∶15， the mass ratio of dosage to PLGA dosage of 6 ∶100, a concentration of polyvinyl alcohol of 10 g/L and an ultrasonic time of 5 minutes. The BDNF-PLGANPs obtained had a diameter of (205±0.18) nm and an EE of (79.65 ± 2.14)%. The release curve shows that the BDNF-PLGA-NPs have a sustained release effect; BDNFPLGANPs can promote the proliferation of ectomesenchymal stem cells in vitro. Conclusion: BDNF-PLGA-NPs prepared by double solvent volatilization method under optimized conditions have high encapsulation rate, good sustained release effect and biological activity.

碳纳米管为石墨六角网平面卷成筒状时套叠而成的新型纳米碳材料，具有高度稳定的管状空腔结构和较大的比表面积，可利用自身吸附或通过化学改性等方式将药物负载在碳纳米管上，成为新型靶向给药系统的研究热点。本文主要从碳纳米管的结构出发，分析其特性和表面功能化的修饰，探究其在药物负载中的应用，并结合碳纳米管材料所面临的问题进行展望。

Objective: To prepare luteolin micelles encapsulated by hyaluronic acid and evaluate its inhibition on lung cancer xenografts in mice. Methods: Reverse phase evaporation method was used to prepare hyaluronic acid-coated luteolin micelles (HA-LE-MC). The prescription was screened with encapsulation efficiency and particle size as optimization indicators, and its apparent morphology，drug loa-ding, encapsulation efficiency and particle size were determined and finally in vivo imaging experiments and in vivo anti-tumor experiments. Results: The best mass ratio of poloxamer 407 (F127) and didecyl dimethyl ammonium bromide (DDAB) was 9 ∶1, and the particle size of HA-LE-MC was about (140.0±1.8) nm, the encapsulation rate was (61.2±2.4) %. The drug loading was (74.0±1.2) μg/mL. In vivo imaging experiments showed that micelles were concentrated in the tumor site after tail vein injection, indicating that micelles had strong tumor targeting. In vivo anti-tumor experiments showed that compared with luteolin HALEMC had stronger inhibitory effect on Lewis lung cancer xenograft tumors (P<0.01), and it had lower side effects compared with the positive drug cisplatin (P<0.05), and could enhance the immune function of tumor-bearing mice. Conclusion: The prepared HA-LE-MC had targeted properties, enhanced antitumor activity and could enhance the immune function of tumor-bearing mice and improve the survival of mice with explanted lung cancer.

目的:  微透析作为近年来新兴的一种活体取样技术，克服了传统取样存在的动物数量较多、前处理过程复杂、不能准确反映成分在机体内实时变化、不能准确测定游离药物浓度等缺点，具有活体、实时、靶向、连续取样等特点，能更真实地反映药物在机体吸收、分布、代谢、排泄的过程，在药动学及药动学药效学（PKPD）结合模型的研究中被广泛应用。本文对微透析技术在药动学及PKPD研究中的应用进行综述，以期为药物的体内过程、作用机制以及靶向性研究提供参考。