Abstract:[Abstract] Objective: To study the effects of RNA interference-mediated silencing of pseudogene Cripto-3(Cr-3) on proliferation and invasion of colon cancer cells. Methods: The cells were divided into five groups, blank control group (Con-A group), empty control group (Con-B group), siRNA transfection group (5 nmol/L group, 10 nmol/L group and 20 nmol/L group). After human colon cancer cell line SW480 were transfected by Cr-3 small interfering RNA (siRNA), the levels of Cr-3 were determined by quantitative real-time PCR. The cell growth and invasion ability was examined by clone formation in soft agar, and Transwell, respectively. Results: Compared with Con-A group and Con-B group, siRNA transfection group (5 nmol/L group, 10 nmol/L group and 20 nmol/L group) had lower levels of Cr-3 mRNA expression with lower invasion ability of colon cancer cells and in a dosedependent manner(both P<0.05). Conclusion:Cr-3 might play an important role in the development of human colon cancer, and down-regulation of Cr-3 by its siRNA could inhibit the invasion of human colon cancer cells.
朱灵, 蒋鹏程, 满昌峰, 彭辉勇, 徐娟, 范钰. RNA干扰假基因Cripto-3表达对大肠癌细胞增殖和侵袭的影响[J]. 江苏大学学报:医学版, 2013, 23(4): 284-287.
ZHU Ling, JIANG Peng-cheng, MAN Chang-feng,et al. RNA interference in pseudogene Cripto-3 on proliferation and invasion of human colon cancer cell. Journal of Jiangsu University(Medicine Edition), 2013, 23(4): 284-287.
[1]Hirotsune S, Yoshida N, Chen A, et al. An expressed pseudogene regulates the messenger-RNA stability of its homologous coding gene[J]. Nature, 2003, 423(6935): 91-96.[2]Poliseno L, Salmena L, Zhang J,et al. A codingindependent function of gene and pseudogene mRNAs regulates tumour biology[J]. Nature, 2010, 465(7301): 1033-1038. [3]KalyanaSundaram S, KumarSinha C, Shankar S, et al. Expressed pseudogenes in the transcriptional landscape of human cancers[J]. Cell, 2012, 149(7):1622-1634.[4]Balasubramanian S, Zheng D, Liu YJ, et al. Comparative analysis of processed ribosomal protein pseudogenes in four mammalian genomes[J]. Genome Biol, 2009, 10(1): R2.[5]Miyoshi N, Ishii H, Mimori K, et al. TDGF1 is a novel predictive marker for metachronous metastasis of colorectal cancer[J]. Int J Oncol, 2010, 36(3):563-568. [6]祁卫东,蒋鹏程,张恒,等. RNAi沉默中期因子基因表达对胃癌细胞黏附和侵袭力的影响[J]. 江苏大学学报:医学版,2010,20(5):369-372.[7]范钰,张尤历,吴莺,等. 大肠癌组织畸胎瘤衍生生长因子1蛋白表达及临床意义[J]. 中华消化内镜杂志,2007,15(33):3484-3488.[8]Zheng D, Gerstein MB. The ambiguous boundary between genes and pseudogenes: the dead rise up, or do they? [J]. Trends Genet, 2007, 23(5): 219-224.[9]de Castro NP, Rangel MC, Nagaoka T, et al. Cripto-1: an embryonic gene that promotes tumorigenesis[J]. Future Oncol, 2010, 6(7):1127-1142.[10]Sun C, Orozco O, Olson DL, et al. CRIPTO3, a presumed pseudogene, is expressed in cancer[J]. Biochem Biophys Res Commun, 2008, 377(1):215-220.[11]Scognamiglio B, Baldassarre G, Cassano C, et al. Assignment of human teratocarcinoma derived growth gactor (TDGF) sequences to chromosomes 2q37, 3q22, 6p25 and 19q13.1[J]. Cytogenet Cell Genet, 1999, 84 ( 3/4 ):220-224.[12]Bianco C, Salomon DS. Targeting the embryonic gene Cripto-1 in cancer and Beyond[J]. Expert Opin Ther Pat, 2010, 20(12):1739-1749.[13]Hentschke M, Kurth I, Borgmeyer U,et al. Germ cell nuclear factor is a repressor of CRIPTO-1 and CRIPTO-3[J].J Biol Chem, 2006, 281(44):33497-33504.