Detection of biofilm formation and biofilm-associated genes of Staphylococcus aureus clinical isolation
(1.School of Medical Science and Laboratory Medicine, Jiangsu University, Zhenjiang Jiangsu 212013; 2.Department of Clinical Laboratory, the Affiliated Hospital of Jiangsu University, Zhenjiang Jiangsu 212001,China)
Abstract：Objective: To detect biofilm formation and biofilmassociated genes of S.aureus clinical isolation. Methods: The biofilm formation was determined by semiquantitative microplate assay and scanning electron microscopy method, and both ica and sarA genes were detected by PCR in 92 strains of S.aureus clinical isolation. Results: All of 92 strains in this study could form biofilm, and carry the sarA gene; 78 strains (84.78%) carried the ica gene. Conclusion: The S.aureus clinical isolations had the ability of biofilm formation, and the gene of ica and sarA were prevalent in S.aureus.
引用本文:
潘红1, 罗欲承1, 赵建忠2, 段秀杰2, 邵世和1. 金黄色葡萄球菌临床分离株生物膜形成能力及其相关基因的检测[J]. 江苏大学学报:医学版, 2012, 22(4): 332-335.
PAN Hong-1, Luo-Yu-Cheng-1, Zhao-Jian-Zhong-2, Duan-Xiu-Jie-2, Shao-Shi-He-1. Detection of biofilm formation and biofilm-associated genes of Staphylococcus aureus clinical isolation. Journal of Jiangsu University(Medicine Edition), 2012, 22(4): 332-335.
2012, Vol. 22 
