Mechanism of miR let-7b inhibiting breast cancer MCF-7 cells migration
（1.Graduate School, 2.Department of CT, the Affiliated Hospital, 3.Department of Tumor Chemoradiation, the Affiliated Hospital, 4.Department of Thoracic Surgery, the Affiliated Hospital of Chengde Medical College, Chengde Hebei 067000, China）
Abstract：Objective: To observe the effect of miR let-7b on inhibiting breast cancer MCF-7 cells migration and to investigate it′s mechanism. Methods: miR let-7b were composed and transfected into breast cancer MCF-7 cells. MiR-control was taken as a reference. The migration of the cells was tested by scratch test and the IL-6 level of MCF-7 cells before and after transfected was tested by PCR and west-blot. Then the combination of miR let-7b and IL-6 was verification by Bioinformatics software and Dual luciferase. Results: Compared with the control group,miR let-7b made the migrating ability of MCF-7 cells reduced,while the anti-miR let-7b strengthen it.Those breast cancer MCF-7 cells which were transfected by miR let-7b had a lower level of IL-6,while those transfected by anti-miR let-7b had a higher level of IL-6.It was verificated by Bioinformatics software and Dual luciferase that miR let-7b was combined with IL-6. Conclusion: miR let-7b inhibited the expression of IL-6 of breast cancer MCF-7 cells at the genetic level and then inhibited the migration.
引用本文:
曲杰1, 王胜林2, 吕喜英3, 李青山3, 杨宗伟4. miR let-7b抑制乳腺癌MCF-7细胞迁移及其机制[J]. 江苏大学学报:医学版, 2012, 22(4): 328-331.
QU Jie-1, WANG Sheng-Lin-2, 吕Xi-Ying-3 , LI Qing-Shan-3, YANG Zong-Wei-4. Mechanism of miR let-7b inhibiting breast cancer MCF-7 cells migration. Journal of Jiangsu University(Medicine Edition), 2012, 22(4): 328-331.
2012, Vol. 22 
