Abstract：Objective: To explore the reliable method of establishing the rat model of gestational diabetes with high fat feed combined with streptozotocin(STZ), and to establish the best dose of stable STZ rat model of gestational diabetes mellitus. Methods: The 6week old SPF rats were fed a highfat diet for 8 weeks after one week of adaptive feeding.According to the ratio of two females to one male, the next day vaginal smear was examined, if the sperm was found D1 day pregnant was recorded. The pregnant rats were randomly divided into control group and STZ groups (15,25,35,45 mg/kg).They were given intraperitoneal injection of the same volume of citric acid buffer (0.1 mol/L, pH=4.2) or STZ 15, 25, 35, 45 mg/kg respectively.Body weight, fasting blood glucose and the rate of moled formation, mortality, offspring were measured or observed. In addition the pancreas, liver and adipose tissue in pregnant rats were assessed by HE staining. Results: The moleding rate of 25 mg/kg group was 62.5%, which was the highest,and the death rate of 12.5% is the lowest.After injection of STZ, blood glucose level of 25,35 and 45 mg/kg groups increased significantly, and the blood level of 25 mg/kg group was more stable. Conclusion: SPF grade 6week old Wistar rats were fed highfat diet for 8 weeks and STZ at the dosage of 25 mg/kg was injected on the first day of gestation, which was a reliable way to establish a stable model of gestational diabetes mellitus in rats.
［1］Hod M, Kapur A, Sacks DA, et al. The International Federation of Gynecology and Obstetrics (FIGO) Initiative on gestational diabetes mellitus: A pragmatic guide for diagnosis, management, and care\[J\]. Int J Gynaecol Obstet, 2015,131(Suppl 3):S173-S211.［2］徐志芳, 杨昱, 陈丽莉, 等. 妊娠期糖尿病发病机制及其对母婴的影响\[J\]. 中国临床医生杂志, 2015,43(8):26-29.［3］Sinzato YK, Volpato GT, Iessi IL, et al. Neonatally induced mild diabetes in rats and its effect on maternal, placental, and fetal parameters\[J\]. Exp Diabetes Res, 2012,2012:108163.［4］李鑫，杨慧霞. 实验性妊娠糖尿病大鼠模型的研究进展\[J\]. 现代妇产科进展, 2012,21(8): 648-650.［5］李佳, 魏波, 塔娜, 等. 不同剂量链脲佐菌素建立妊娠期糖尿病大鼠模型稳定性的比较\[J\]. 第四军医大学学报, 2009,30(22):2500-2502.［6］蒋静，吴春维，李兴，等. 不同剂量链脲佐菌素建立妊娠糖尿病大鼠模型的研究\[J\]. 贵阳医学院学报, 2010,35(3):221-223.［7］Herrera E，Palacin M，Martin A，et al. Relationship between maternal and fetal fuels and placental glucose transfer in rats with maternal diabetes of varying severity\[J\]. Diabetes, 1985, 32(Suppl 2):42-46.［8］庄璟怡，应豪，王德芬. 小剂量链脲佐菌素建立妊娠期糖尿病大鼠模型的研究\[J\]. 现代妇产科进展, 2014,23(8):607-610.［9］Szkudelski T. The mechanism of alloxan and streptozotocin action in B cells of the rat pancreas\[J\]. Physiol Res, 2001,50(6):537-546.［10］Sivitz WI. Lipotoxicity and glucotoxicity in type 2 diabetes.Effects on development and progression\[J\]. Postgrad Med, 2001,109(4):55-59，63-64.［11］HAPO Study Cooperative Research Group, Metzger BE, Lowe LP, et al. Hyperglycemia and adverse pregnancy outcomes\[J\]. N Engl J Med, 2008,358(19):1991-2002.
2018, Vol. 28 
