Abstract：[Abstract] Objective: To investigate the effect of lipopolysaccharide(LPS) on the proliferation,apoptosis, phagocytosis and reactive oxygen species(ROS) production in thyroid follicular cells(TFCs) in vitro, and to determine the pathologic damage of TFCs in response to LPS. Methods: Nthy-ori 3-1 cells were incubated with gradient concentrations of 0, 10, 20, 100, 150 ng·mL-1 LPS for 48 h and with 100 ng·mL-1 for 24 h or 48 h, MTT assay was used to measure cell proliferation. Nthy-ori 3-1 cells were treated with 100 ng·mL-1 LPS for 48 h, and flow cytometry was used to measure cell apoptosis. Nthy-ori 3-1 cells were incubated with gradient concentrations of 0, 10, 20, 100 ng·mL-1 LPS for 12 h, and Nile red fluorescent FluoSpheres beads uptake method was used to measure phagocytosis of cells. Nthy-ori 3-1 cells were treated with gradient concentrations of 0, 10, 20, 100 ng·mL-1 LPS for 4 h, and fluorescent probe DCFH-DA was used to measure ROS level of cells. Results: Compared with controls, 100 ng·mL-1 LPS promoted the proliferation and significantly increased the level of ROS of Nthy-ori 3-1 cells(P<0.01). In addition, 20 ng·mL-1 and 100 ng·mL-1 LPS promoted the phagocytic function of Nthy-ori 3-1 cells(P<0.01). However, 100 ng·mL-1 LPS did not increase the apoptosis of Nthy-ori 3-1cells(P>0.05). Conclusion: LPS promoted the pathologic damage of TFCs through up-regulation of the proliferation, phagocytosis and the level of intracellular ROS of TFCs, suggesting that LPS may play an important role in the development of Hashimoto′s thyroiditis.
［Key words］thyroid follicular cells; phagocytosis; lipopolysaccharide; reactive oxygen species
罗旋, 牟笑, 郑婷婷, 董昕, 刘宝翠, 毛朝明. 脂多糖对甲状腺上皮细胞增殖、吞噬及胞内活性氧产生的影响[J]. 江苏大学学报:医学版, 2008, 28(03): 222-226.
LUO Xuan, Mou-Xiao, Zheng-Ting-Ting, Dong-Xin, Liu-Bao-Cui, Mao-Chao-Ming. Effects of LPS on the proliferation, phagocytosis and ROS production in thyroid follicular cells in vitro. Journal of Jiangsu University(Medicine Edition), 2008, 28(03): 222-226.
2008, Vol. 28 
