Effects and mechanism of exogenous IL-33 on cardiac fibroblast collagen expression under diabetic condition in vitro
WANG Hao1， TAO Ai-bin1, ZHANG Guo-hui1， RUI Tao1, CHEN Yong-chang2
(1. Department of Cardiology, the Affiliated People′s Hospital of Jiangsu University, Zhenjiang Jiangsu 212002； 2.School of Medicine, Jiangsu University, Zhenjiang Jiangsu 212013，China)
Abstract：Objective： To investigate the role of IL-33 on cardiac fibrosis under high glucose treatment on cardiac fibroblasts. Methods：Isolated rat cardiac fibroblasts were divided into following groups: control (DMEM/F12+30 mmol/L mannitol), high glucose(DMEM/F12+30 mmol/L glucose),high glucose+HMGB1(1 μg/mL), high glucose +HMGB1+IL-33 (3 ng/mL).The expression of the collagen I, IL-33 and DGK ζ in the cardiac fibroblasts were evaluated by western blotting. The DAG and the activity of PKCβ was detected by ELISA. Results：When cardiac fibroblasts challenged with high glucose, the collagen I generation was increased and the production of IL-33 was decreased. The effect of high glucose was exaggerated by HMGB1. In addition, the DGKζ expression was decreased, DAG content was increased and further promoted the activation of PKCβ.Exogenous IL-33 attenuated above effects. Conclusion：Exogenous IL-33 plays a key role in anti-cardiac fibrosis induced by high glucose.
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2016, Vol. 26 
