Abstract：Objective: To investigate the antitumor activity and life extention effect of Clinacanthus nutans (Burm.f.) Lindau n-butanol extract (CN-N) on tumor loaded mice. Methods:Mice hepatoma carcinoma cells (Heps, grown in donor mice) were transplanted subcutaneously into armpit of the ICR mice to establish the tumor xenografts model. Tumor mice were randomized into four groups: control group, cytoxan group, CN-N low dose group (3 mg·kg-1·d-1), CN-N high dose group (10 mg·kg-1·d-1). Tumor inhibition rate, body weights, immune organs index and life prolonging effect were recorded. Proliferation of tumor cells were detected by western blotting and apoptosis was examined by TUNEL assay. Results: Compared with the control group, CN-N low dose group and high dose group showed remarkable inhibition of tumor weight (t=2.261, 3.140, both P＜0.05);no significant differences on spleen and thymus index of tumor earing mice(both P＞0.05). PCNA expression levels were greatly reduced when treated with low or high concentration of CN-N. Compared with the control group, CN-N high dose group significantly extended the survival time(t=3.416, P=0.003 1). Conclusion: CN-N exhibited good antitumor activity and significantly prolonged the survival time of the tumor-bearing mice.
通讯作者:
高静（通讯作者），教授，博士生导师，E-mail:jinggao@ujs.edu.cn
作者简介: 刘旭（1988—），男，硕士研究生
引用本文:
刘旭1, 郭文洁1, 黄丹民1, 2, 高静1. 鳄嘴花正丁醇提取物对小鼠Heps肝癌的抑制作用[J]. 江苏大学学报:医学版, 2014, 24(3): 211-.
LIU Xu-1, Guo-Wen-Jie-1, Huang-Dan-Min-1, 2 , Gao-Jing-1. Inhibitive effect of Clinacanthus nutans (Burm.f.) Lindau n-butanol extracts on Heps hepatoma in mice. Journal of Jiangsu University(Medicine Edition), 2014, 24(3): 211-.
