Diosgenin affect human gastric cancer BGC-823 and SGC-7901 cells through MAPK pathways
WU Yuan-yuan1， CUI Guo-xing1， MA Tie-liang2， DING Wei-liang2， GE Zhi-jun3， TANG Zhi-an4
（1.Department of Gastroenterology,2.Central Laboratory，3.Department of Critical Care Medicine，4.Department of Traditional Chinese Medicine，the Affiliated Yixing Hospital of Jiangsu University，Yixing Jiangsu 214200，China）
Abstract：Objective: To discuss the role of diosgenin in the proliferation, invasion，migration and apoptosis of human gastric cancer BGC-823 and SGC-7901 cells via MAPK signaling pathways. Methods: Human gastric cancer cell lines BGC-823 and SGC-7901 were cultured in vitro and treated with diosgenin. Proliferation rate, cell migration and invasion were measured by MTT method via Transwell assay. And protein expression of apoptosis-associated protein (AAP) BAX, apoptosis inhibitor protein Bcl-2 and Erk1/2,JNK and p38 proteins of MAPK pathways were measured by Western Blot. Results: When BGC-823 and SGC-7901 cells were treated with diosgenin，the proliferation, migration and invasion of BGC-823 and SGC-7901 cells were significantly decreased. The apoptosis of both cells was enhanced to a certain extent. As to the correlation of gastric cancer cells and MAPK pathways，we found that p-p38 protein expression level after diosgenin treated was dramatically down-regulated; however，the expression levels of Erk1/2，JNK，p38，p-Erk1/2 and p-JNK were negligible. Conclusion: Diosgenin might affect the proliferation，invasion，migration and apoptosis of human gastric cancer BGC-823 and SGC-7901 cells through p-p38 of MAPK pathways.
作者简介: 吴媛媛(1986—)，女，江苏宜兴人，医师，主要从事胃肠肿瘤研究。
引用本文:
吴媛媛1, 崔国兴1, 马铁梁2, 丁伟良2, 葛志军3 , 唐志安4. 薯蓣皂苷元对人胃癌BGC-823和SGC-7901细胞生物学行为的影响[J]. 江苏大学学报:医学版, 2014, 24(3): 207-.
WU Yuan-Yuan-1, Cui-Guo-Xing-1, Ma-Tie-Liang-2, Ding-Wei-Liang-2, Ge-Zhi-Jun-3 , Tang-Zhi-An-4. Diosgenin affect human gastric cancer BGC-823 and SGC-7901 cells through MAPK pathways. Journal of Jiangsu University(Medicine Edition), 2014, 24(3): 207-.
