薯蓣皂苷元体内外抑制胃癌增殖的机制

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摘要目的: 研究薯蓣皂苷元抑制胃癌增殖及转移的主要途径及其机制。方法: 分别使用1,10,100 μg/mL浓度薯蓣皂苷元处理人血管内皮细胞(human umbilical vein endothelial cells,HUVEC)和胃癌BGC823细胞，制作细胞生长曲线，观察薯蓣皂苷元对细胞增殖能力的影响；选取一定浓度的薯蓣皂苷元处理HUVEC和BGC823细胞，体外成管实验和划线法分别检测药物处理对血管内皮细胞成管能力及肿瘤细胞迁移能力的影响。将BGC823细胞接种到裸鼠制作皮下肿瘤模型，连续静脉注射薯蓣皂苷元，分析比较其对皮下肿瘤的抑制效果。结果：与对照组比较，薯蓣皂苷元可明显抑制内皮细胞增殖活性（P＜0.05），且具有浓度依赖性；而相同处理方式对BGC823细胞的增殖活性基本无影响；10 μg/mL的薯蓣皂苷元对BGC823细胞的迁移能力具有明显的抑制作用，能够明显抑制HUVEC细胞体外成管能力；与模型组比较，薯蓣皂苷元给药组肿瘤抑制率明显增高（达38.2%），差异有统计学意义（P＜0.05）。结论：薯蓣皂苷元可以通过抑制内皮细胞增殖活性影响肿瘤血供，并最终抑制皮下肿瘤模型的增殖，同时，其可通过直接的作用抑制胃癌的迁移。

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关键词 ：薯蓣皂苷元, 人血管内皮细胞, BGC-823细胞, 增殖活性, 体外成管, 迁移, 裸鼠成瘤

Abstract：Objective: To investigate the principal pathway and preliminary mechanism by which diosgenin inhibits gastric carcinoma in vivo by treatment of human umbilical vein endothelial cells(HUVEC) and gastric carcinoma(BGC823) cells for comparison. Methods: HUVEC and BGC823 cells were treated with diosgenin at different concentrations and cell growth curves were plotted to observe the effects of diosgenin on proliferative activities of endothelial cells and gastric carcinoma cells; the scraping line method and canalization in vitro were used to detect the effects of diosgenin treatment on canalization of endothelial cells and migration ability of gastric carcinoma cells by treating HUVEC and BGC823 cells with diosgenin at a certain concentration;a subcutaneous tumor model was established by inoculating nude mice with BGC823 cells and diosgenin was administrated continuously to analyze its inhibitory effects on subcutaneous tumors. Results: Diosgenin had a significant effect on proliferation of endothelial cells in a concentrationdependent way, with a significant difference between the treatment groups and the control group at 48 or 72 h(P<0.05);and the same treatment had no evident effect on the proliferative activity of BGC823 cells and effectively inhibited the migration of BGC823 cells and significantly reduced the canalization of HUVEC cells in vitro; diosgenin exhibited a good inhibitory effect on subcutaneous tumors in the nude mice model of BGC823 cells at an inhibitory rate of 38.2%(P<0.05) in comparison with the model group. Conclusion: Diosgenin can eventually inhibit the proliferation of the subcutaneous tumor model by suppressing endothelial cell activity and consequently suppressing blood supply.

收稿日期: 2014-07-29

作者简介: 霍中华（1970—），男，安徽太和人，主任医师，博士，主要从事消化道肿瘤的诊治研究。

引用本文:

霍中华，胡君，储著陵，吕胜，尹鹏，章丽. 薯蓣皂苷元体内外抑制胃癌增殖的机制[J]. 江苏大学学报:医学版, 2014, 24(05): 394-398. HUO Zhong-hua, HU Jun, CHU Zhu-ling, LV Sheng, YIN Peng, ZHANG Li. Mechanism underlying inhibition of migration of gastric carcinoma by diosgenin in vitro and vivo . Journal of Jiangsu University(Medicine Edition), 2014, 24(05): 394-398.

链接本文:

http://zzs.ujs.edu.cn/xbyxb/CN/ 或 http://zzs.ujs.edu.cn/xbyxb/CN/Y2014/V24/I05/394

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