Abstract: According to the monotonic loading experiment of three specimens, the static behavior of steel-concrete composite beams prestressed with external tendons was investigated. The results show that adding prestressing with external tendons can effectively improve the bending capacity of composite beams with reduced ductility at the same time. Strain distribution at the cross section in pure flexure zone remains approximately linear. Increasing the spacing of studs welded on steel beams can improve ultimate deformability of prestressed beams with slight effect on bending capacity. With the decrease of shear connection degree, the slip of studs increases at ultimate load. According to different finite element models of composite beams prestressed with external tendons, the effects of concrete compressive strengthen, effective prestress, external tendons profile and studs spacing on the static behavior of composite beams were discussed. A simplified calculation method of bending capacity was proposed based on prestress effect to provide references for the design and calculation of composite beams prestressed with external tendons.