Abstract: To solve the problems of autonomous underwater vehicle (AUV) with high test cost, requiring ideal simulation environment for the application of underwater robots and robot verification and difficult modeling of simulation environment, the MOOS software system was used to perform navigation, communication and threedimensional simulation of the AUV navigation system. The communication methods, the software architecture, the data collection methods and the sensor data types of the MOOS software system were configured and simulated. Based on the perspectives of mechanism, the data subscription and publication and the data analysis and processing, the MOOS software system was analyzed and verified. The results show that the MOOS system can generate relevant environmental sensor data according to the preset trajectory of AUV, which can effectively realize multisource information fusion and navigation pose calculation.