Abstract: To investigate the effects of subcritical, ultrasonic and organic solvent methods on the extraction of wheat germ oil, the corresponding moisture content, yield, extraction rate, acid value, peroxide value, anisidine value, saponification value, composition of fatty acid and Vitamin E (VE) were measured. The results show that yield, extraction rate, saponification value, unsaturated fatty acid and αVE are the highest by subcritical extraction technology with the values of (10.37±0.12)%, (90.52±0.12)%, 176.19 mg·g-1, 86.70% and 9 252.20 mg·kg-1,respectively. However, acid value, peroxide value and anisidine value are the least with the values of 8.95 mg·g-1, 2.18 mmol·kg-1 and 0.46, respectively. Yield, extraction rate, saponification value, unsaturated fatty acid and αVE are the least by organic solvent extraction with the values of (7.78±0.15)%, (67.89±0.15)%, 159.89 mg·g-1, 75.80% and 7 526.00 mg·kg-1,respectively. However, acid value, peroxide value and anisidine value are the highest with the values of 11.37 mg·g-1, 3.18 mmol·kg-1 and 0.96, respectively. The extraction technology has obvious effect on wheat germ oil quality , and the quality of wheat germ oil is the best by the subcritical extraction technology.