Preparation and application of epoxidized magnetic silica nanoparticles
1.School of Food and Biological Engineering, Jiangsu University, Zhenjiang, Jiangsu 212013, China； 2.School of Food Engineering, Henan Vocational College of Agriculture, Zhengzhou, Henan 451450， China
Abstract：Fe3O4 magnetic nanoparticles were modified by surfactants of polyethylene glycol 6000 (PEG 6000) and sodium dodecyl benzene sulfonate (SDBS). The epoxidized magnetic silica nanoparticles were obtained by sol-gel method and ethanol-acid method. Lysozymes was immobilized on the epoxidized magnetic silica nanoparticles. The results show that the enzyme recovery is 78.6 % with lysozyme loading of 114.9 mg?g-1 when the immobilizing time is 2 h with buffer of pH 8.0 and initial lysozyme concentration of 1.0 g?L-1. The immobilized lysozymes have improved storage stability.
2016, Vol. 37 
