Abstract：A production method of high hydroxyl radical inhibition by water-soluble protein from stress millet was developed. The test indicated that H2O2 and Protamex play key roles in the hydroxyl radical inhibitory activity. Fractional factorial design, central composite experimental design and response surface methodology were used to optimize those factors. The optimal stress medium composition was listed as follows ： H2O2 1.56 % , gibberellin 0. 259 6 mg/L, Protamex 0. 248 g/L and Tween -40 0.7%. With the optimal medium, the highest hydroxyl radical inhibition （58.16%） was achieved.
李景军, 陈正行, 毛健. 抑制羟自由基的粟水溶性蛋白胁迫培养基试验[J]. 江苏大学学报（自然科学版）, 2008, 29(1): 9-12.
Li Jingjun, Chen Zhengxing, Mao Jian. Optimization of medium enforced hydroxyl radical inhibition by water-soluble protein from stress millet[J]. Journal of Jiangsu University(Natural Science Eidtion)
, 2008, 29(1): 9-12.
2008, Vol. 29 
