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Effects of α-lipoic acid on liver oxidative stress and endoplasmic reticulum stress in type 2 diabetic rats |
| LI Jing1, LI Rui-fang2 , WANG Guo-xian1, LI Yun2 |
(1.Department of Pharmacology，Liaoning Medical College, Jinzhou Liaoning 121001；2.Department of Cerebral Surgery, the Hospital of Chinese Medicine of Handan，Handan Hebei 056001, China） |
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Abstract Objective: To investigate the protective effects of α-lipoic acid(A-LA) on liver in type 2 diabetic rats；the JNK and p-JNK protein expression related to ERS of liver in 2 diabetic rat. Method：Type 2 diabetes mellitus models were induced by highfat diet and intraperitoneal injection of streptozotocin in fifty male Sprague Dawley rats. then divided into three groups: diabetic group, A-LA group and Vit E group.The groups except diabetic group treated with A-LA and VitE by intragastric for twelve weeks. The fasting glucose，blood lipid and serum liver function indices were measured after twelve weeks. Apoptosis was detected by TdT-mediated dUTP nick end labeling；Western blot analysis was employed to detect liver Phospho-c-Jun／JNK protein expression. Results: Compared with the control groups, diabetes model rats blood sugar and TG,TC,AST,ALT and MDA increased significantly (P<0.05), SOD and GSH-Px reduced significantly (P<0.05), the rate of cell apoptosis was increased. Protein expression of JNK didn′t not change but phosphorylated JNK were significantly up (P<0.05). Compared with the diabetic group, A-LA groups above all indexes had been improved, the activities of SOD and GSH-Px were improved; the rate of cell apoptosis was reduced, protein expression of p-JNK was down. Vit E group blood sugar was not changed,but the SOD and GSH-Px increased significantly. Conclusion： Alpha lipoic acid has a protective effect on liver in diabetic rats, the mechanism may be related to the inhibition of oxidative stress and endoplasmic reticulum stress
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Received: 08 October 2012
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2013, Vol. 23 
