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Effects of α-lipoic acid on liver oxidative stress and endoplasmic reticulum stress in type 2 diabetic rats |
LI Jing1, LI Rui-fang2 , WANG Guo-xian1, LI Yun2 |
(1.Department of Pharmacology,Liaoning Medical College, Jinzhou Liaoning 121001;2.Department of Cerebral Surgery, the Hospital of Chinese Medicine of Handan,Handan Hebei 056001, China) |
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Abstract Objective: To investigate the protective effects of α-lipoic acid(A-LA) on liver in type 2 diabetic rats;the JNK and p-JNK protein expression related to ERS of liver in 2 diabetic rat. Method:Type 2 diabetes mellitus models were induced by highfat diet and intraperitoneal injection of streptozotocin in fifty male Sprague Dawley rats. then divided into three groups: diabetic group, A-LA group and Vit E group.The groups except diabetic group treated with A-LA and VitE by intragastric for twelve weeks. The fasting glucose,blood lipid and serum liver function indices were measured after twelve weeks. Apoptosis was detected by TdT-mediated dUTP nick end labeling;Western blot analysis was employed to detect liver Phospho-c-Jun/JNK protein expression. Results: Compared with the control groups, diabetes model rats blood sugar and TG,TC,AST,ALT and MDA increased significantly (P<0.05), SOD and GSH-Px reduced significantly (P<0.05), the rate of cell apoptosis was increased. Protein expression of JNK didn′t not change but phosphorylated JNK were significantly up (P<0.05). Compared with the diabetic group, A-LA groups above all indexes had been improved, the activities of SOD and GSH-Px were improved; the rate of cell apoptosis was reduced, protein expression of p-JNK was down. Vit E group blood sugar was not changed,but the SOD and GSH-Px increased significantly. Conclusion: Alpha lipoic acid has a protective effect on liver in diabetic rats, the mechanism may be related to the inhibition of oxidative stress and endoplasmic reticulum stress
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Received: 08 October 2012
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[1] 谷川久一,秦丽娟.氧化应激与肝病[J].日本医学介绍,2006,27(12):560-562. [2] Maya L, Villarreal FJ. Diagnostic approaches for diabetic cardiomyopathy and myocardial fibrosis[J]. J Mol Cell Cardiol, 2010, 48(3): 524-529. [3] Harmancey R, Taegtmeyer H. The complexities of diabetic cardiomyopathy: lessons from patients and animal models[J]. Curr Diab Rep, 2008, 8(3): 243-248. [4] 杨义生,陈家伦.内质网应激和2型糖尿病J.中国糖尿病杂志,2006,14(5):393-395. [5] 吴柳,范竹萍.糖尿病与肝病的研究进展[J].国际消化病杂志,2008,28(4):326-329. [6] 陈杰,李甘地.病理学[M].北京:人民卫生出版社,2006:18-155. [7] Portincasa P,Grattagliano I,Palmieri VO,et al.Current pharmacological treatment of nonalcoholic fatty liver[J].Curr Med Chem,2006,13(24):2889-2900. [8] 刘芸野,谢青.活性氧与内质网应激介导肝细胞凋亡研究进展[J].肝脏,2006,11(4):281-283. [9] Eizirik DL,Cardozo AK,Cnop M,et al.The role for endoplasmic reticulum stress in diabetes mellitus[J].Endocr Rev,2008,29(1):42-61. [10] DiazDelfin J,Morales M,Caelles C,et al. Hypoglycemic action of Thiazolidines diones/peroxisome proliferator-activated receptor gamma by inhibition of the c-Jun NH2-terminal kinase pathway[J].Diabetes,2007,56(7):1865-1871. [11] 李安林, 施用晖, 岳鹏,等. 硫辛酸对高脂日粮大鼠脂类代谢和抗氧化能力的影响[J].食品科学,2006,27(5):242-245. |
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