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| Bioinformatics analysis of human ferredoxin 1, a key regulatory gene for cuproptosis |
| GUAN Yunliang1,2 |
(1. Basic Medical College, 2. Brain Science Research Center, Panzhihua University, Panzhihua Sichuan 617000, China)
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Abstract Objective： To analyze the gene structure and characteristics of ferredoxin 1 (FDX1), a key regulatory gene for cuproptosis, using bioinformatics methods. Methods： The human FDX1 DNA sequence was obtained from GenBank database, and bioinformatics analysis of FDX1 general characteristics, structural recognition, characteristic analysis, and tissue expression was conducted by using data mining software. Results： The human FDX1 gene is located at 11q22.3, with a total length of 35 553 bp, strong codon bias, and the repetitive sequence accounting for 61.40% of the total length of the gene. There are 5 promoters (score>0.90), 71 transcription factor binding sites and polyadenylation tail signal at site 518 in the upstream 2 000 bp region, and no CpG island can be predicted. Subcellular localization of FDX1 is most likely in the mitochondria. The molecular function prediction showed that FDX1 had 2Fe-2S cluster binding activity. The maximum mRNA of human FDX1 gene expression was in the adrenal gland ［146(100×FPKM)1/2］. The cancer specificity of FDX1 mRNA in each tissue is low. Conclusion： The mitochondrial subcellular localization, 2Fe-2S cluster activity, and involved signaling pathways of FDX1 have been confirmed that FDX1 could be used as a key regulatory genes for cuproptosis from the perspective of biological algorithms.
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Received: 19 July 2022
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