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p53 lactylation promotes the proliferation and metastasis of colon cancer cells |
DAI Yao1, DAI Haiyan1, DA Wenxin1, WANG Yahui1, ZHANG Ziqi2, WANG Shengjun1, MA Jie1#br# |
(1. School of Medicine, Jiangsu University, Zhenjiang Jiangsu 212013; 2. Clinical Laboratory, The Fourth Affiliated People′s Hospital of Jiangsu University, Zhenjiang Jiangsu 212001, China)
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Abstract Objective: To investigate the effect and mechanism of p53 lactylation on the proliferation and metastasis of colon cancer cells. Methods: Human colon cancer tissues and corresponding adjacent normal tissues were collected, and the pathological morphology was confirmed by hematoxylin-eosin staining. Lactate concentration was detected by lactate detection kit, and the expression of lactylation and p53 were detected by immunohistochemical staining. The subcellular localization of lactylation and p53 in HCT116 cells were observed by immunofluorescence, and p53 lactylation was detected by immunocoprecipitation. Subsequently, endogenous lactate was used to mediate p53 lactylation of HCT116 cells and the lactate inhibitor oxamate was used to inhibit p53 lactylation. The proliferation ability of HCT116 cells was detected by CCK-8. The migration and invasion ability of HCT116 cells were detected by scratch and Transwell assay. Results: The expression of lactylation and p53 in human colon cancer tissue was higher than that in adjacent normal colon cancer tissue, and p53 lactylation was clearly observed. Endogenous lactate in HCT116 cells can mediate p53 lactylation, inhibit p53 entry into the nucleus, and promote cell proliferation,migration and invasion. Conclusion: p53 lactylation promotes proliferation and metastasis of colon cancer cells by inhibiting the nuclear translocation of p53.
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Received: 13 December 2022
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