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| comThe role of circ_0000515/miR-1296-5p/CDK2 axis in breast cancer |
| YANG Qin1#, WANG Chao2#, LIN Bohan2, YU Qiwen2, LI Tao2, SUN Xiaochun2 |
(1. Women′s Health Department, the Fourth Affiliated Hospital of Jiangsu University, Zhenjiang Jiangsu 212002; 2. School of Medicine, Jiangsu University, Zhenjiang Jiangsu 212013, China)
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Abstract Objective： To explore the role and underlying mechanism of circ_0000515 in the progression of breast cancer. Methods： The circRNAs chip dataset (GSE101123) of breast cancer was downloaded from GEO database and analyzed by R language. The target circRNA was selected and its expression in breast cancer cell lines and tissues were detected. The target miRNA of the circRNA and the target mRNA of the miRNA were selected by bioinformatics analysis, and verified by double luciferase experiment and Western blotting. The siRNA of circRNA was transfected into MCF-7 and MDA-MB-231. The cell proliferation and migration were measured by CCK-8 and scratch assay. The expression of RNA were detected by qRT-PCR. The expression of mRNAs-encoded protein was detected by Western blotting. Results： GSE101123 dataset was selected and downloaded. Five highly expressed circRNAs molecules were screened by R language, and circ_0000515 was selected as the research object for subsequent study. Bioinformatics analysis showed that there may be a targeted relationship among circ_0000515, miR12965p and cell cycledependent kinase 2 (CDK2). qRT-PCR results showed that the expression of circ_0000515 in breast cancer cell lines was higher than that in normal cell lines, and its expression in cancer tissues was higher than that in paracancerous tissues. After knockdown the expression of circ_0000515, the proliferation and migration ability of breast cancer cells decreased significantly, while the expression of miR-1296-5p increased. Double luciferase assay confirmed that there was a targeted relationship between circ_0000515 and miR-1296-5p. Western blotting showed that the expression of CDK2 decreased after knocking down the expression of circ_0000515. Conclusion： circ_0000515 is highly expressed in breast cancer. circ_0000515/miR-1296-5p/CDK2 axis may play a role in the progression of breast cancer.
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Received: 25 November 2022
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