小鼠骨髓间质干细胞来源的胞外囊泡对尼古丁诱导的小鼠生精细胞损伤的修复作用

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Abstract Objective: To construct an in vitro model of nicotine-induced injury of mouse spermatogenic cells and to explore the repairing effect of mouse bone marrow mesenchymal stem cellderived extracellular vesicles (mBMMSCsEVs) on the nicotine-induced damage of spermatogenic cells GC-1. Methods: Different concentrations (0, 8, 16, 32 μg/mL) of nicotine were used to treat spermatogenic cell GC-1 for 24, 48, 72 h, respectively; and the optimal concentration of nicotine and time of treatment to induce damage on GC-1 cells were screened by MTT assay. The spermatogenic cells GC-1 were divided into control group, nicotine group and nicotine+extracellular vesicle group. MTT, Transwell and apoptosis experiments were used to evaluate the changes in the biological characteristics of damaged GC-1 cell before and after the intervention of mBMMSCsEVs. Results: The results of MTT assay showed that the proliferation of GC-1 was significantly decreased when the concentration of nicotine was 32 μg/mL for 24 h (P<0.05), mBMMSCs-EVS increased cell proliferation after treatment with nicotine (P<0.05); compared with the control group, GC-1 cells in nicotine group showed significantly reduced migration and increased apoptosis(P<0.01); compared with the nicotine group, nicotine+extracellular vesicle group showed significantly increased cell proliferation and migration, and decreased the apoptotic ability(P<0.05 or<0.01). Conclusion: mBMMSCs-EVs may repair the damage of nicotine on the proliferation and migration of spermatogenic cells and inhibited nicotine-induced apoptosis of spermatogenic cells.

Received: 04 March 2021

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	Articles by authors
	LIU Ziyao1
	HUANG Yue2
	WANG Chao1
	LI Tao1
	WANG Jingzhe1
	LIN Bohan1
	WANG Zixuan1
	SUN Xiaochun1
	CHEN Huabiao3

Cite this article:

LIU Ziyao1,HUANG Yue2,WANG Chao1等.

Repairing effect of mouse bone marrow mesenchymal stem cell-derived extracellular vesicles on nicotineinduced damage of mouse spermatogenic cells

[J]. Journal of Jiangsu University(Medicine Edition), 2022, 22(1): 44-49.

URL:

http://zzs.ujs.edu.cn/xbyxb/EN/ OR http://zzs.ujs.edu.cn/xbyxb/EN/Y2022/V22/I1/44

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