食管癌细胞来源的外泌体促进人脐静脉血管内皮细胞血管生成

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Abstract Objective: To explore the promotion effects of exosome from human esophageal cancer cells EC109 （EC109-ex） on the tube formation of human umbilical vein endothelial cells （HUVECs）. Methods: EC109ex was isolated from the supernatant of cultured EC109. EC109ex was characterized in terms of surface markers CD9 and CD63 by Western blotting and morphology by transmission electron microscopes （TEM）. HUVECs were treated with EC109ex （5 μg/mL and 10 μg/mL） or PBS （control group）, respectively. Then invasion and tube formation of HUVECs were examined using Transwell and Matrigel assay. Expression of vascular specific marker CD31, CD34 and VEGF were examined by Western blotting．Results: EC109ex located in the cytoplasm of HUVECs. Compared with control group，EC109-ex increased the migration efficiency (P<0.01) and number of tube formation of HUVECs （P<0.01）．Furthermore, EC109ex treatment increased expression of vascular specic markers of CD31, CD34 and VEGF in HUVECs（P<0.01）. Conclusion: Esophageal cancer cells could promote tumor angiogenesis through exosome mediated paracrine action.

Received: 08 December 2020

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	Articles by authors
	SHI Jinsheng
	WANG Maosong
	LI Benzhong
	ZHANG Xiumei

Cite this article:

SHI Jinsheng,WANG Maosong,LI Benzhong等.

Human esophageal cancer cells derived exosomes enhance the angiogenesis of human umbilical vein endothelial cells

[J]. Journal of Jiangsu University(Medicine Edition), 2021, 31(05): 412-416，420.

URL:

http://zzs.ujs.edu.cn/xbyxb/EN/ OR http://zzs.ujs.edu.cn/xbyxb/EN/Y2021/V31/I05/412

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