|
|
|
|
Effect of Morroniside on the activation and differentiation of T lymphocytes in mice |
|
WU Yi, QIU Xin, ZHANG Yan, ZHOU Jing-wen, LI Ci, ZHAO Chuan-xiang, GAO Feng-wei, LIU Shuo, YAO Xue, XIA Sheng |
| (School of Medicine, Jiangsu University, Zhenjiang Jiangsu 212013, China) |
|
|
|
|
Abstract Objective: To study the effect of Cornus officinalis extract Morroniside on T lymphocytes and its potential mechanisms. Methods: The spleen of C57 BL/6 mice was used to prepare single cell suspension, which were activated with antiCD3 and antiCD28 stimulation. Splenocytes were divided into control group and Morroniside group(treated with 10 μmol/L Morroniside). The activation (CD69, CD25), proliferation and apoptosis in both CD4+ and CD8+ T cells were detected. Moreover, the differentiation proportions of CD4+T cell subsets were analyzed by flow cytometry. CD4+T cell subsets were analyzed by flow cytometry. The mRNA level of Tbx21, Gata3, lymphoid enhance factor 1(LEF1) in cells were detected by realtime fluorescence quantitative PCR. The protein expression of β-catenin was analyzed by Western blotting. Results: Compared with the control group, the proportions of activated T lymphocytes differentiated into Th1, Th2 and Treg were significantly increased in the morroniside group (P<0.05). Although the activation and proliferation marker CD25 and Ki67 were enhanced, there were no significant difference in the proportions of proliferation and apoptosis between the two groups. The cytokine levels of IL-4 secreted by Th2 cells and TGF- β related to Treg cells were also markedly increased (P<0.05). IFNγ secreted by Th1 cells was not statistically significant. The mRNA levels of Tbx21, Gata3, LEF1 and the protein expression of βcatenin were increased in the Morroniside group (P<0.05). Conclusion: Morroniside extracted from Cornus officinalis could promote the differentiation of T lymphocytes, which may be related with Wnt signaling pathway.
|
|
Received: 21 March 2019
|
|
|
|
| ［1］Czerwin＇ska ME, Melzig MF. Cornus mas and Cornus OfficinalisAnalogies and differences of two medicinal plants traditionally used\[J\]. Front Pharmacol, 2018, 9: 894.［2］Dong Y, Feng ZL, Chen HB, et al. Corni Fructus: a review of chemical constituents and pharmacological activities\[J\]. Chin Med, 2018, 13: 34.［3］Huang Y, Wang S, Liu L, et al. Review of traditional uses, botany, chemistry, pharmacology, pharmacokinetics, and toxicology of Radix Cyathulae\[J\]. Chin Med, 2019, 14: 17.［4］Park JY, Han AR, Kil YS, et al. A new secoiridoid glycoside from the fruits of Cornus officinalis (Cornaceae)\[J\]. Nat Prod Res, 2016, 30(13): 1504-1510.［5］Clevers H. Wnt/βcatenin signaling in development and disease\[J\]. Cell, 2006, 127(3): 469-480.［6］Zhou L, Wang H, Jing J, et al. Morroniside regulates hair growth and cycle transition via activation of the Wnt/βcatenin signaling pathway\[J\]. Sci Rep, 2018, 8(1): 13785.［7］Pi WX, Feng XP, Ye LH, et al. Combination of Morroniside and Diosgenin prevents high glucoseinduced cardiomyocytes apoptosis\[J\]. Molecules, 2017, 22(1): pii:E163.［8］Li M, Wang W, Wang P, et al. The pharmacological effects of Morroniside and loganin isolated from Liuweidihuang Wan, on MC3T3E1 cells\[J\]. Molecules, 2010, 15(10): 7403-7414.［9］Xu Y, Banerjee D, Huelsken J, et al. Deletion of βcatenin impairs T cell development\[J\]. Nat Immunol, 2003, 4(12): 1177-1182.［10］Xue T, Roy R. Studying traditional Chinese medicine\[J\]. Science, 2003, 300(5620): 740-741.［11］Park CH, Noh JS, Kim JH, et al. Evaluation of morroniside, iridoid glycoside from Corni Fructus, on diabetesinduced alterations such as oxidative stress, inflammation, and apoptosis in the liver of type 2 diabetic db/db mice\[J\]. Biol Pharm Bull, 2011, 34(10): 1559-1565.［12］Sung YH, Chang HK, Kim SE, et al. Antiinflammatory and analgesic effects of the aqueous extract of corni fructus in murine RAW 264.7 macrophage cells\[J\]. J Med Food, 2009, 12(4): 788-795.［13］Famili F, Naber BA, Vloemans S, et al. Discrete roles of canonical and noncanonical Wnt signaling in hematopoiesis and lymphopoiesis\[J\]. Cell Death Dis, 2015, 6: e1981.［14］Ma J, Wang R, Fang X, et al. βcatenin/TCF1 pathway in T cell development and differentiation\[J\]. J Neuroimmune Pharmacol, 2012, 7(4): 750-762.［15］Xu HQ, Hao HP, Zhang X, et al. Morroniside protects cultured human umbilical vein endothelial cells from damage by high ambient glucose\[J\]. Acta Pharmacol Sin, 2004, 25(4): 412-415.［16］Sato A, Kayama H, Shojima K, et al. The Wnt5aRor2 axis promotes the signaling circuit between interleukin12 and interferonγ in colitis\[J\]. Sci Rep, 2015, 5: 10536.［17］Yu Q, Sharma A, Oh SY, et al. T cell factor 1 initiates the T helper type 2 fate by inducing the transcription factor GATA3 and repressing interferonγ\[J\]. Nat Immunol, 2009, 10(9): 992-999. |
|
|
|
2019, Vol. 29 
