肝细胞癌患者内质网应激凋亡信号通路的性别差异肝细胞癌患者内质网应激凋亡信号通路的性别差异

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Abstract Objective: To investigate the effect of gender on endoplasmic reticulumstress(ERstress) and apoptosis signals in patients with hepatocellular carcinoma. Methods: Clinical data was collected and retrospectively analyzed from 80 routine hepatocellular carcinoma(HCC) patients, including 40 male and 40 female. Normal liver parenchymal tissues were obtained as controls from 10 Chinese patients with hemangioma.The clinical typing of tumors was determined according to the TNM classification system. The histological grade of tumor differentiation was evaluated by the Edmondson grading system and liver function was assessed by ChildPugh score system. Characteristics of ERstress and apoptosis signal in patients and controls were analyzed by Western blotting. Results: The levels of ERstress signal factors including glucose regulated protein 78(GRP78),GRP94,spliced X boxbinding protein 1(sXBP1), activating transcription factor 6(ATF6),inositol requiring enzyme 1(IRE1α),C/EBP homologous protein(CHOP) were elevated significantly compared with control(P＜0.05). Meanwhile, the level of Caspase3 was increased in HCC patients.The clinic features,such as stage, HBV infection, liver cirrhosis and Child grade had on obvious effect on ERstress signal.Only had age affected on GRP78 levels rather than other ERstress signal factors. Interestingly,the levels of sXBP1, ATF6, CHOP and Caspase3 elevated significantly, while GRP78 level reduced in male patients compared with those of the female(P＜0.05). ATF6 was also reduced significantly in poorlydifferentiated patients(P＜0.05).Further studies indicated that differentiation had no effect on ERstress and apoptosis signals. Conclusion: ERstress and apoptosis signals were induced and GRP78 level was increased in male HCC patients. These results provided a preliminary data for personalized therapy based on gender.

Received: 27 June 2017

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http://zzs.ujs.edu.cn/xbyxb/EN/ OR http://zzs.ujs.edu.cn/xbyxb/EN/Y2017/V27/I5/432

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