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| Low molecular weight heparin attenuates acute lung injury induced by lipopolysaccharide in rats |
| LIU Yu1, LIU Shao-zheng1, WANG Xian-feng1, WANG Yi-qiao2, LI Yuan-hai2 |
| (1. Department of Anesthesiology, Wanbei CoalElectric Group General Hospital, Suzhou Anhui 234011; 2. Department of Anesthesiology, the First Affiliated Hospital of Anhui Medical University, Hefei Anhui 230032, China) |
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Abstract Objective： To explore the effect of low molecular weight heparin(LMWH) on acute lung injury(ALI) induced by lipopolysaccharide(LPS) in rats. Methods： Fiftysix male SD rats(body weight 250～300 g) were randomly divided into control group(n=8), ALI group(n=24) and ALI+LMWH group(n=24). ALI was induced by intraperitoneal injection of LPS(6 mg/kg). ALI+LMWH group and control group received intraperitoneal injection of LPS(6 mg/kg)+LMWH(450 U·kg-1), the same volume of normal saline, respectively. Arterial blood gases, activated partial thromboplastin time(APTT), prothrombin time(PT), ratio of wet lung to dry lung(W/D), the levels of myeloperoxidase(MPO), malondialdehyde(MDA) in lung tissues and levels of interleukin-6(IL-6) and tumor necrosis factor-α(TNF-α) in serum and bronchoalveolar lavage fluid(BALF) were determined 2, 4 and 6 h after injection of LPS or normal saline in both groups, respectively. Results： There was significant difference in each index between control group and ALI group(P<0.05); compared with ALI group, arterial blood gases were significantly improved in ALI+LMWH group, lung W/D ratio, MDA and MPO in the lung tissue and the levels of TNF-α, IL-6 in serum and BALF were all significantly decreased(P<0.05); compared with control group, APTT and PT in ALI group were shortened, while delayed in ALI+LMWH group(P<0.05). Conclusion： LMWH could decrease cytokine, ameliorate systemic inflammation in LPS induced ALI, which may be resulted from inhibition of anticoagulant activity.
［Key words］lipopolysaccharide; acute lung injury; low molecular weight heparin; anticoagulant
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Received: 23 September 2016
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2016, Vol. 26 
