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| Inhibitory effect of the ginsenoside Rb-2 on replication of enterovirus 71 in vitro
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| JI Yun 1,2, CHEN Yong-chang 1, MA Jin-hong 2 |
| （1. School of Medicine,Jiangsu University,Zhenjiang Jiangsu 212013; 2. Department of Laboratory Medicine, First People′s Hospital of Changzhou, Changzhou Jiangsu 213001, China) |
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Abstract Objective: To investigate the inhibitory effect of ginsenosides Rb-2 on enterovirus 71(EV71). Methods: The cytotoxicity of ginsenosides Rb-2 on human embryonic rhabdomyosarcoma(RD) cells was detected by MTT method.The inhibitory effect of ginsenosides Rb-2 on EV71 was observed by cytopathic effect method, and the influence of the drug on direct inactivating and absorbing effect was also studied. Results: When ginsenosides Rb-2 was at a concentration of ≤100 μg/mL,there was no significant cytotoxicity on RD cells. At 6.25, 12.5, 25, 50, 75 and 100 μg/mL, the inhibitory rates of ginsenosides Rb-2 against EV71 were (16.0±2.8)%,(23.4±2.5)%,(61.4±3.1)%, (77.9±2.9)%,(98.3±0.8)% and (98.6±1.0)%,respectively.The 50% inhibitory concentration was 20.8 μg/mL.Treated by the mixture of ginsenosides Rb-2 (≤100μg/mL) and EV71,the direct inactivating effect on EV71 wasn′t found in RD cells. When both ginsenosides Rb-2（75 μg/mL）and EV71 were added, or added at 1 hr postinfection, there was no statistical significance in viral titers. Conclusion: Ginsenosides Rb-2 can effectively inhibit EV71 replication.
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Received: 25 October 2014
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2016, Vol. 26 
