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| Establishment of enterovirus 71-infected ICR neonatal mice model |
| QIN Yan-yan1, LU Jie1, LI Ji-lin1, LIU Hai-bing2, MAO Ling-xiang2, SHAO Qi-xiang1, CHEN Jian-guo2 |
(1. School of Medicine, Jiangsu University, Zhenjiang Jiangsu 212013; 2. Department of Clinical Laboratory, the Affiliated People′s Hospital of Jiangsu University, Zhenjiang Jiangsu 212002, China) |
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Abstract Objective: To establish an EV71 infection model in ICR neonatal mice and provide a practical animal model for evaluation of EV71 vaccines and antiviral drugs. Methods: The 1day ICR mice were infected with the EV71 G03 virus strain through intracerebroventricular injection to establish animal models. After routine euthanizingly killed animals, their various organs and tissues were collected to perform RTPCR analysis of the viral nucleotide acids. In addition, pathological observation, immunohistochemical staining and the Western blotting were used to identify the expression of viral protein 1(VP1). Results: Pathological observation found that after infection, specific pathological changes, such as bleeding, inflammatory cell infiltration, appeared in the myocardium tissue, lung tissue, skeletal muscle, small intestine, brain tissue and other organs of mice. Immunohistochemical staining showed that all five tissues of the infected mice had EV71 virusspecific antigen distribution. Western blotting analysis demonstrated that EV71 VP1 protein was expressed in all five tissues. Conclusion: The onedayold ICR neonatal EV71infected mouse model was successfully established.
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Received: 21 April 2017
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2017, Vol. 27 
